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Comparison of single and dual platform methodologies for the estimation of CD34+ hematopoietic progenitor cells: correlation with colony assay
- Source :
- ResearcherID
- Publication Year :
- 2002
-
Abstract
- In this study three assays for the enumeration of CD34+ progenitors were compared: 1) a modified version of the Milan protocol, used in the standard dual-platform format; 2) a dual-platform version of the ISHAGE protocol; 3) the ProCOUNT software version 2.0/ProCOUNT kit. The assays were compared to validate the accuracy of CD34+ cell counts in mobilized peripheral blood (PB), apheresis products (AP), and cord blood (CB). The ProCOUNT protocol uses reference beads for absolute CD34+ cell counting, whereas CD34 counts by other techniques are derived from a separate leukocyte count performed by a hematology analyzer. A good correlation between the ISHAGE and ProCOUNT methods was obtained for estimation of CD34+ counts in PB (n=42 samples analyzed) and AP (n=35) - except for samples having a leukocyte count >25 x 10 9 /L or a CD34 count
- Subjects :
- Adult
Cancer Research
Pathology
medicine.medical_specialty
Adolescent
Clinical Biochemistry
CD34
Antigens, CD34
Biology
Pathology and Forensic Medicine
Flow cytometry
medicine
Enumeration
Humans
flow cytometry
hematopoietic stem cells
transplantation
hematology
oncology
Progenitor cell
Clonogenic assay
Chromatography
medicine.diagnostic_test
short term colony assay
Middle Aged
Fetal Blood
Hematopoietic Stem Cells
Cell counting
Blood Cell Count
Transplantation
Oncology
Cord blood
hematopoietic progenitor cells
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- ResearcherID
- Accession number :
- edsair.doi.dedup.....95f8119dd79991b6354913cab39713de