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Targeted Measurements of O- and N-Glycopeptides Show That Proteins in High Density Lipoprotein Particles Are Enriched with Specific Glycosylation Compared to Plasma
- Source :
- Journal of proteome research, vol 17, iss 2
- Publication Year :
- 2018
- Publisher :
- eScholarship, University of California, 2018.
-
Abstract
- High density lipoprotein (HDL) particles are believed to be protective due to their inverse correlation with the prevalence of cardiovascular diseases. However, recent studies show that in some conditions such as heart disease and diabetes, HDL particles can become dysfunctional. Great attention has been directed toward HDL particle composition because the relative abundances of HDL constituents determine HDL's functional properties. A key factor to consider when studying the structure and composition of plasma particles is the protein glycosylation. Here, we profile the O- and N-linked glycosylation of HDL associated-proteins including the truncated form of Apo CIII and their glycan heterogeneity in a site-specific manner. Apolipoprotein CIII, fetuin A, and alpha 1 antitrypsin are glycoproteins associated with lipoproteins and are implicated in many cardiovascular and other disease conditions. A targeted method (UHPLC-QQQ) was used to measure the glycoprotein concentrations and site-specific glycovariations of the proteins in human plasma and compared with HDL particles isolated from the same plasma samples. The proteins found in the plasma are differentially glycosylated compared to those isolated in HDL. The results of this study suggest that glycosylation may play a role in protein partitioning in the blood, with possible functional implications.
- Subjects :
- 0301 basic medicine
Glycan
Biochemistry & Molecular Biology
Glycosylation
HDL
alpha-2-HS-Glycoprotein
Lipoproteins
Apo CIII
Cardiovascular
Biochemistry
03 medical and health sciences
chemistry.chemical_compound
High-density lipoprotein
Tandem Mass Spectrometry
Humans
Cluster Analysis
Amino Acid Sequence
Peptide sequence
fetuin A
Protein Processing
chemistry.chemical_classification
Chromatography
Apolipoprotein C-III
UPLC
biology
Post-Translational
glycopeptides
General Chemistry
Biological Sciences
Atherosclerosis
Fetuin
030104 developmental biology
chemistry
Carbohydrate Sequence
high density lipoprotein
alpha 1-Antitrypsin
High Pressure Liquid
Chemical Sciences
biology.protein
lipids (amino acids, peptides, and proteins)
MRM
Glycoprotein
alpha-2-HS-glycoprotein
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Journal of proteome research, vol 17, iss 2
- Accession number :
- edsair.doi.dedup.....95fad4fdfd09b6bc5ff40c2e7ee0c348