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Ubiquitin-fold modifier 1 inhibits apoptosis by suppressing the endoplasmic reticulum stress response in Raw264.7 cells
- Source :
- International Journal of Molecular Medicine. 33:1539-1546
- Publication Year :
- 2014
- Publisher :
- Spandidos Publications, 2014.
-
Abstract
- Ubiquitin-fold modifier 1 (Ufm1) is a new member of the ubiquitin-like protein family, and its biological function remains largely unknown, particularly in macrophages. In this study, we demonstrate that Ufm1 expression is increased in diabetic mouse resident peritoneal macrophages (RPMs) and in the mouse macrophage cell line, Raw264.7, subjected to endoplasmic reticulum (ER) stress. Following treatment of the cells with the ER stress inducers, thapsigargin (TG) or tunicamycin (TM), the lentiviral short hairpin RNA (shRNA)-mediated knockdown of Ufm1 increased the apoptosis of Raw264.7 cells. Furthermore, these cells had higher expression levels of immunoglobulin heavy chain-binding protein (BiP) and C/EBP homologous protein (CHOP), which are markers of the ER stress response. The overexpression of Ufm1 induced by lentiviral infection in the Raw264.7 cells treated with the ER stress inducers, TG or TM, resulted in the opposite effect. Taken together, our results suggest that Ufm1 is upregulated in diabetic mouse RPMs and in Raw264.7 cells in response to ER stress and that Ufm1 protects macrophages against apoptosis. Thus, Ufm1 may be a novel gene that protects against ER stress-induced apoptosis in macrophages.
- Subjects :
- Thapsigargin
Macrophages
Endoplasmic reticulum
Proteins
Apoptosis
General Medicine
Tunicamycin
Biology
Endoplasmic Reticulum Stress
Cell Line
Cell biology
Small hairpin RNA
chemistry.chemical_compound
Downregulation and upregulation
chemistry
Cell culture
Genetics
Unfolded protein response
Animals
RNA, Small Interfering
Endoplasmic Reticulum Chaperone BiP
Heat-Shock Proteins
Transcription Factor CHOP
Subjects
Details
- ISSN :
- 1791244X and 11073756
- Volume :
- 33
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Medicine
- Accession number :
- edsair.doi.dedup.....95fd99d9d45acd05c7d5cdf126d8cb9f