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Characterization of the human MANB gene encoding lysosomal alpha-D-mannosidase
- Source :
- Gene. 198(1-2)
- Publication Year :
- 1997
-
Abstract
- Genomic clones of human MANB gene encoding the lysosomal enzyme, alpha-mannosidase, have been isolated, sequenced and analyzed. The human MANB gene spans approximately 22 kb and consists of 24 exons. The 5' flanking region of the gene shows a high G+C content and has two Sp1 and three AP-2 sites. Promoter analysis using deletion constructs of the 5' flanking region fused to the bacterial CAT gene showed that 150 bp of 5' sequence could drive the expression of MANB in COS 7 cells. Determination of the sequence of the 5' end of the alpha-mannosidase mRNA by 5' RACE protocol showed that transcription is initiated from a cluster of sites centered -28 and -20 bp from the first in-frame ATG. These data demonstrate that, like other lysosomal enzyme genes such as those for beta-glucuronidase or beta-hexosaminidase, the human MANB gene is controlled by a short 5' flanking sequence located near the initiation codon.
- Subjects :
- Transcription, Genetic
Molecular Sequence Data
Restriction Mapping
Biology
Exon
Restriction map
Start codon
Bacterial Proteins
Transcription (biology)
Multienzyme Complexes
Genetics
Humans
Amino Acid Sequence
Promoter Regions, Genetic
Gene
Peptide sequence
Messenger RNA
Mannose-6-Phosphate Isomerase
Base Sequence
Intron
General Medicine
Exons
Molecular biology
Nucleotidyltransferases
Introns
Genes
Lysosomes
Subjects
Details
- ISSN :
- 03781119
- Volume :
- 198
- Issue :
- 1-2
- Database :
- OpenAIRE
- Journal :
- Gene
- Accession number :
- edsair.doi.dedup.....96103dbcfbbc1b5a0879403c580b473c