Back to Search
Start Over
microRNA‑630 promotes cell proliferation and inhibits apoptosis in the HCT116 human colorectal cancer cell line
- Source :
- Molecular medicine reports. 16(4)
- Publication Year :
- 2016
-
Abstract
- Dysregulation of microRNAs (miRNAs) in colorectal cancer provides important opportunities for the development of future miRNA‑based therapies. The present study aimed to assess the role of miRNA‑630 (miR‑630) expression in colorectal cancer. HCT116 human colorectal cancer cells were transfected with miR‑630 inhibitor, mimic or control miRNA, and the effects of miR‑630 dysregulation on cell viability, proliferation and apoptosis were analyzed using MTT and bromodeoxyuridine assays, and an annexin V‑fluorescein isothiocyanate cell apoptosis kit, respectively. In addition, the changes in the protein expression of proliferation‑associated and AKT signaling pathway proteins were analyzed by western blot analysis. The results of the present study demonstrated that overexpression of miR‑630 significantly promoted HCT116 cell proliferation however inhibited apoptosis. Furthermore, miR‑630 overexpression reduced the protein expression of p27, BCL2‑associated X apoptosis regulator, procaspase‑3 and active caspase‑3, and increased the levels of phosphorylated‑AKT and BCL2 apoptosis regulator. The suppression of miR‑630 led to the opposite results. In conclusion, the present findings suggested that miR‑630 may function as an oncogenic miRNA in colorectal cancer, and may promote cellular proliferation and inhibit apoptosis, through the regulation of the expression of p27 and the AKT signaling pathway. The present study suggested that the inhibition of miR‑630 may have potential as an alternative therapeutic strategy for the treatment of patients with colorectal cancer.
- Subjects :
- 0301 basic medicine
Cancer Research
Cell Survival
Apoptosis
Biology
medicine.disease_cause
Biochemistry
03 medical and health sciences
0302 clinical medicine
microRNA
Genetics
medicine
Humans
Molecular Biology
Cell Proliferation
Oncogene
Apoptosis Regulator
Akt/PKB signaling pathway
Cancer
medicine.disease
HCT116 Cells
Gene Expression Regulation, Neoplastic
MicroRNAs
030104 developmental biology
Oncology
030220 oncology & carcinogenesis
Cancer research
Molecular Medicine
RNA Interference
Signal transduction
Carcinogenesis
Colorectal Neoplasms
A431 cells
Proto-Oncogene Proteins c-akt
Signal Transduction
Subjects
Details
- ISSN :
- 17913004
- Volume :
- 16
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Molecular medicine reports
- Accession number :
- edsair.doi.dedup.....962fa56a9cd992b3d90fee7389fede94