Back to Search
Start Over
Direct recognition of LPS by human but not murine CD8+T cellsviaTLR4 complex
- Source :
- European Journal of Immunology. 39:1564-1572
- Publication Year :
- 2009
- Publisher :
- Wiley, 2009.
-
Abstract
- LPS comprises a major PAMP and is a key target of the immune system during bacterial infection. While LPS can be recognised by innate immune cells via the TLR4 complex, it is unknown whether T lymphocytes, especially CD8(+) T cells are also capable of doing so. We report here that naive human CD8(+) T cells, after activation by TCR stimulation, express surface TLR4 and CD14. These activated CD8(+) T cells can then secrete high concentrations of IFN-gamma, granzyme and perforin in response to LPS. These effects can be specifically inhibited using siRNA for TLR4. Furthermore, LPS can synergize with IL-12 to polarize the CD8(+) T cells into cytotoxic T-cell 1 (Tc1) that produce IFN-gamma but not IL-4, with or without TCR activation. Moreover, CD8(+)CD45RO(+) memory T cells constitutively expressed TLR4 and markedly enhanced IFN-gamma production when stimulated with LPS. In contrast, activated murine CD8(+) T cells lack TLR4 and CD14 expression and fail to respond to LPS for proliferation and cytokine production. Thus, human but not murine CD8(+) T cells are able to directly recognise bacterial LPS via LPS receptor complex and TLR4 provides a novel signal for the activation of effector and memory human CD8(+) T cells.
- Subjects :
- Lipopolysaccharides
CD3 Complex
Immunology
Lipopolysaccharide Receptors
Lymphocyte Antigen 96
Gene Expression
CD8-Positive T-Lymphocytes
Biology
Lymphocyte Activation
Antibodies
Granzymes
Interferon-gamma
Mice
Interleukin 21
Immune system
T-Lymphocyte Subsets
Animals
Humans
Immunology and Allergy
Cytotoxic T cell
IL-2 receptor
RNA, Small Interfering
Cell Proliferation
Mice, Inbred C3H
Perforin
Tumor Necrosis Factor-alpha
ZAP70
Interleukin-12
Mice, Mutant Strains
Toll-Like Receptor 2
Cell biology
Toll-Like Receptor 4
Granzyme
Interleukin 12
biology.protein
Leukocyte Common Antigens
lipids (amino acids, peptides, and proteins)
CD8
T-Lymphocytes, Cytotoxic
Subjects
Details
- ISSN :
- 15214141 and 00142980
- Volume :
- 39
- Database :
- OpenAIRE
- Journal :
- European Journal of Immunology
- Accession number :
- edsair.doi.dedup.....9632f2ad59ab84da9898aecd5c239f01