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Pyruvate-sensitive AOX exists as a non-covalently associated dimer in the homeothermic spadix of the skunk cabbage, Symplocarpus renifolius
- Source :
- FEBS letters. 581(30)
- Publication Year :
- 2007
-
Abstract
- The cyanide-resistant alternative oxidase (AOX) is a homodimeric protein whose activity can be regulated by the oxidation/reduction state and by α-keto acids. To further clarify the role of AOX in the skunk cabbage, Symplocarpus renifolius, we have performed expression and functional analyses of the encoding gene. Among the various tissues in the skunk cabbage, SrAOX transcripts were found to be specifically expressed in the thermogenic spadix. Moreover, our data demonstrate that the SrAOX protein exists as a non-covalently associated dimer in the thermogenic spadix, and is more sensitive to pyruvate than to other carboxylic acids. Our results suggest that the pyruvate-mediated modification of SrAOX activity plays a significant role in thermoregulation in the skunk cabbage.
- Subjects :
- Thermogenic plants
Pyruvate
Alternative oxidase
Dimer
Blotting, Western
Cell Respiration
Molecular Sequence Data
Submitochondrial Particles
Biophysics
Carboxylic Acids
Flowers
Mitochondrion
Biochemistry
Dithiothreitol
Mitochondrial Proteins
chemistry.chemical_compound
Structural Biology
Gene Expression Regulation, Plant
biology.animal
Pyruvic Acid
Genetics
Homeothermy
Araceae
Amino Acid Sequence
RNA, Messenger
Molecular Biology
Gene
Plant Proteins
Diamide
Skunk cabbage
biology
Respiration
Temperature
Cell Biology
NAD
humanities
Mitochondria
chemistry
Ketoglutaric Acids
Spadix (botany)
Skunk
Oxidoreductases
Dimerization
Oxidation-Reduction
Subjects
Details
- ISSN :
- 00145793
- Volume :
- 581
- Issue :
- 30
- Database :
- OpenAIRE
- Journal :
- FEBS letters
- Accession number :
- edsair.doi.dedup.....966eb0374bbc01a1706fba28630c7052