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Phosphorylation of the RecQ Helicase Sgs1/BLM Controls Its DNA Unwinding Activity during Meiosis and Mitosis
- Source :
- Developmental Cell, 53 (6), Developmental Cell
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- Summary The Bloom’s helicase ortholog, Sgs1, orchestrates the formation and disengagement of recombination intermediates to enable controlled crossing-over during meiotic and mitotic DNA repair. Whether its enzymatic activity is temporally regulated to implement formation of noncrossovers prior to the activation of crossover-nucleases is unknown. Here, we show that, akin to the Mus81-Mms4, Yen1, and MutLγ-Exo1 nucleases, Sgs1 helicase function is under cell-cycle control through the actions of CDK and Cdc5 kinases. Notably, however, whereas CDK and Cdc5 unleash nuclease function during M phase, they act in concert to stimulate Sgs1 activity during S phase/prophase I. Mechanistically, CDK-mediated phosphorylation enhances the velocity and processivity of Sgs1, which stimulates DNA unwinding in vitro and joint molecule processing in vivo. Subsequent hyper-phosphorylation by Cdc5 appears to reduce the activity of Sgs1, while activating Mus81-Mms4 and MutLγ-Exo1. These findings suggest a concerted mechanism driving orderly formation of noncrossover and crossover recombinants in meiotic and mitotic cells.
- Subjects :
- Saccharomyces cerevisiae Proteins
DNA repair
RecQ helicase
Mitosis
Cell Cycle Proteins
Saccharomyces cerevisiae
Protein Serine-Threonine Kinases
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
0302 clinical medicine
Cyclin-dependent kinase
Phosphorylation
DNA, Fungal
Homologous Recombination
Molecular Biology
030304 developmental biology
0303 health sciences
RecQ Helicases
biology
Helicase
Cell Biology
Processivity
Cell biology
Meiosis
biology.protein
Homologous recombination
Protein Processing, Post-Translational
030217 neurology & neurosurgery
Developmental Biology
Sgs1
Subjects
Details
- ISSN :
- 15345807
- Volume :
- 53
- Database :
- OpenAIRE
- Journal :
- Developmental Cell
- Accession number :
- edsair.doi.dedup.....9684f5908315f829719effff907f1cc5