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Serotonergic neurons in the nucleus raphé obscurus are not involved in the ventilatory and thermoregulatory responses to hypoxia in adult rats
- Source :
- Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual), Universidade de São Paulo (USP), instacron:USP
- Publication Year :
- 2013
- Publisher :
- Elsevier BV, 2013.
-
Abstract
- The medullary raphé is an important component of the central respiratory network, playing a key role in CO2 central chemoreception. However, its participation in hypoxic ventilatory responses is less understood. In the present study, we assessed the role of nucleus raphé obscurus (ROb), and specifically 5-HT neurons confined in the ROb, on ventilatory and thermoregulatory responses to hypoxia. Chemical lesions of the ROb were performed using either ibotenic acid (non-specific lesion; control animals received PBS) or anti-SERT-SAP (5-HT specific lesion; control animals received IgG-SAP). Ventilation (V˙E; whole body plethysmograph) and body temperature (Tb; data loggers) were measured during normoxia (21% O2, N2 balance) and hypoxia exposure (7% O2, N2 balance, 1h) in conscious adult rats. Ibotenic acid or anti-SERT-SAP-induced lesions did not affect baseline values of V˙E and Tb. Similarly, both lesion procedures did not alter the ventilatory or thermoregulatory responses to hypoxia. Although evidence in the literature suggests a role of the rostral medullary raphé in hypoxic ventilatory responses, under the present experimental conditions our data indicate that caudal medullary raphé (ROb) and its 5-HT neurons neither participate in the tonic maintenance of breathing nor in the ventilatory and thermal responses to hypoxia.
- Subjects :
- Male
Pulmonary and Respiratory Medicine
Serotonin
medicine.medical_specialty
Time Factors
Microinjections
Physiology
Cell Count
Biology
Serotonergic
Antibodies
Nucleus raphe obscurus
Body Temperature
Lesion
chemistry.chemical_compound
Internal medicine
Excitatory Amino Acid Agonists
medicine
Animals
Rats, Wistar
Hypoxia
Ibotenic Acid
Plethysmography, Whole Body
Raphe
General Neuroscience
RNA-Binding Proteins
Thermoregulation
Rats
Disease Models, Animal
Endocrinology
chemistry
MODELOS ANIMAIS
Anesthesia
Breathing
Raphe Nuclei
Blood Gas Analysis
medicine.symptom
Pulmonary Ventilation
Ibotenic acid
Body Temperature Regulation
Serotonergic Neurons
Subjects
Details
- ISSN :
- 15699048
- Volume :
- 187
- Database :
- OpenAIRE
- Journal :
- Respiratory Physiology & Neurobiology
- Accession number :
- edsair.doi.dedup.....96ad8e6c89a1d18a265d558933265734
- Full Text :
- https://doi.org/10.1016/j.resp.2013.04.008