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Intestinal Microbiota in Patients with Spinal Cord Injury
- Source :
- PLoS ONE, Vol 11, Iss 1, p e0145878 (2016), PLoS ONE
- Publication Year :
- 2016
- Publisher :
- Public Library of Science (PLoS), 2016.
-
Abstract
- Human intestinal flora comprises thousands of bacterial species. Growth and composition of intestinal microbiota is dependent on various parameters, including immune mechanisms, dietary factors and intestinal motility. Patients with spinal cord injury (SCI) frequently display neurogenic bowel dysfunction due to the absence of central nervous system control over the gastrointestinal system. Considering the bowel dysfunction and altered colonic transit time in patients with SCI, we hypothesized the presence of a significant change in the composition of their gut microbiome. The objective of this study was to characterize the gut microbiota in adult SCI patients with different types of bowel dysfunction. We tested our hypothesis on 30 SCI patients (15 upper motor neuron [UMN] bowel syndrome, 15 lower motor neuron [LMN] bowel syndrome) and 10 healthy controls using the 16S rRNA sequencing. Gut microbial patterns were sampled from feces. Independent of study groups, gut microbiota of the participants were dominated by Blautia, Bifidobacterium, Faecalibacterium and Ruminococcus. When we compared all study groups, Roseburia, Pseudobutyrivibrio, Dialister, Marvinbryantia and Megamonas appeared as the genera that were statistically different between groups. In comparison to the healthy group, total bacterial counts of Pseudobutyrivibrio, Dialister and Megamonas genera were significantly lower in UMN bowel dysfunction group. The total bacterial count of Marvinbryantia genus was significantly lower in UMN bowel dysfunction group when compared to the LMN group. Total bacterial counts of Roseburia, Pseudobutyrivibrio and Megamonas genera were significantly lower in LMN bowel dysfunction group when compared to healthy groups. Our results demonstrate for the first time that butyrate-producing members are specifically reduced in SCI patients when compared to healthy subjects. The results of this study would be of interest since to our knowledge, microbiome-associated studies targeting SCI patients are non-existent and the results might help explain possible implications of gut microbiome in SCI. Copyright © 2016 Gungor et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
- Subjects :
- Male
0301 basic medicine
Blautia
lcsh:Medicine
Gut flora
Pseudobutyrivibrio
Gastroenterology
Feces
0302 clinical medicine
RNA, Ribosomal, 16S
Ruminococcus
lcsh:Science
Spinal cord injury
Phylogeny
Bifidobacterium
Spinal cord
Intestine flora
Multidisciplinary
Ribosome RNA
Intestines
Spinal Cord
Megamonas
Female
Roseburia
Species dominance
Research Article
Dialister
Adult
medicine.medical_specialty
food.ingredient
RNA 16S
Firmicutes
RNA sequence
Case-control studies
Inflammatory bowel diseases
Biology
Microbiology
03 medical and health sciences
food
Neurogenic Bowel
Internal medicine
medicine
Humans
Motor neuron disease
Microbiome
Motor Neuron Disease
Gastrointestinal microbiome
Spinal Cord Injuries
Faecalibacterium
Feces analysis
Marvinbryantia
lcsh:R
Inflammatory Bowel Diseases
biology.organism_classification
medicine.disease
Gastrointestinal Microbiome
030104 developmental biology
Case-Control Studies
lcsh:Q
Neurogenic bowel
Bacterial load
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 11
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....96d7c6f94d049b7f28663338eb758386