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Lactulose drives a reversible reduction and qualitative modulation of the faecal microbiota diversity in healthy dogs
- Source :
- Scientific Reports, Ferreira, M, Salavati Schmitz, S, Schoenebeck, J, Clements, D, Campbell, S, Gaylor, D, Mellanby, R, Gow, A & Salavati, M 2019, ' Lactulose drives a reversible reduction and qualitative modulation of the faecal microbiota diversity in healthy dogs ', Scientific Reports, vol. 9, 13350 . https://doi.org/10.1038/s41598-019-50090-7, Scientific Reports, Vol 9, Iss 1, Pp 1-11 (2019)
- Publication Year :
- 2019
- Publisher :
- Nature Publishing Group UK, 2019.
-
Abstract
- Hepatic encephalopathy is a frequent and debilitating complication of liver disorders. Lactulose is an established and reasonably effective treatment, yet with incompletely understood mechanisms of action. The aims of this study were to examine how the faecal microbiota composition changed before, during and after lactulose treatment in a large animal model. Healthy, privately owned dogs (n = 18) completed a prospective cohort study. Faecal samples were collected weekly, while the subjects were either on their usual diet (week 1), or a standardised diet (weeks 2–9), with added oral lactulose in weeks 6–7. DNA extraction and 16S rRNA gene sequencing were undertaken. Faecal samples from week 7 had a significantly lower microbiota richness/diversity, based on observed operational taxonomic units, Shannon/Chao1 indexes and Pielou’s Evenness. Beta diversity based on UniFrac distances was significantly different in week 7 compared to weeks 1, 5 and 9. At the phylum level, week 7 was associated with a significant increase of Firmicutes and Actinobacteria, and a decrease of Bacteroidetes and Fusobacteria, when compared to weeks 5 and 9. In summary, we have shown that lactulose induces a reversible qualitative and quantitative change of the faecal microbiota, which may explain its clinical efficacy in the management of hepatic encephalopathy.
- Subjects :
- 0301 basic medicine
DNA, Bacterial
Male
Firmicutes
lcsh:Medicine
Physiology
Article
Actinobacteria
Microbial ecology
03 medical and health sciences
Lactulose
Feces
0302 clinical medicine
Dogs
Gastrointestinal Agents
RNA, Ribosomal, 16S
medicine
Animals
Prospective Studies
lcsh:Science
Prospective cohort study
Hepatic encephalopathy
2. Zero hunger
Multidisciplinary
biology
Bacteria
business.industry
lcsh:R
Bacteroidetes
High-Throughput Nucleotide Sequencing
Fusobacteria
Biodiversity
biology.organism_classification
medicine.disease
3. Good health
Gastrointestinal Microbiome
UniFrac
030104 developmental biology
Hepatic Encephalopathy
Liver cirrhosis
Dysbiosis
lcsh:Q
030211 gastroenterology & hepatology
Female
business
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....96dd0e3df1dd18755c2b351d19ca9049
- Full Text :
- https://doi.org/10.1038/s41598-019-50090-7