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Dual Effects of Alpha-Arbutin on Monophenolase and Diphenolase Activities of Mushroom Tyrosinase
- Source :
- PLoS ONE, PLoS ONE, Vol 9, Iss 10, p e109398 (2014)
- Publication Year :
- 2014
- Publisher :
- Public Library of Science, 2014.
-
Abstract
- The effects of α-arbutin on the monophenolase and diphenolase activities of mushroom tyrosinase were investigated. The results showed that α-arbutin inhibited monophenolase activity but it activated diphenolase activity. For monophenolase activity, IC50 value was 4.5 mmol · L(-1) and 4.18 mmol · L(-1) of α-arbutin could extend the lag time from 40.5 s to 167.3 s. Alpha- arbutin is proposed to be regarded as a triphenolic substrate by the enzyme during catalyzation, leading to the suicide inactivation of the active site of tyrosinase. For diphenolase activity, α-arbutin acted as an activator and its activation mechanism was mixed type activation. To reveal such activation, it should be mainly refered to the conformational changes in tyrosinase caused by the interaction of α-arbutin with residues located at the entrance to the active site, and the decrease of the effect of suicide inactivation.
- Subjects :
- Tyrosinase
lcsh:Medicine
Bioengineering
Biochemistry
Protein Chemistry
Melanin
chemistry.chemical_compound
Chemical Biology
Biomechanics
lcsh:Science
chemistry.chemical_classification
Multidisciplinary
biology
Chemistry
Activator (genetics)
Plant Biochemistry
Monophenol Monooxygenase
Arbutin
lcsh:R
Substrate (chemistry)
Active site
Biology and Life Sciences
Biochemical Reactivation
Biochemical Activity
Enzyme structure
Enzyme
biology.protein
Biocatalysis
Enzymology
lcsh:Q
Agaricales
Oxidoreductases
Research Article
Biotechnology
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 9
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....96e3411773c8f45f82467b98daeb2239