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Storage of Porcine Cornea in an Innovative Bioreactor
- Source :
- Investigative Opthalmology & Visual Science. 58:5907
- Publication Year :
- 2017
- Publisher :
- Association for Research in Vision and Ophthalmology (ARVO), 2017.
-
Abstract
- Purpose To quantify cell survival and tissue structure preservation of porcine cornea stored in a bioreactor (BR) that recreates a transcorneal pressure gradient equivalent to intraocular pressure (IOP) and renews the medium. Methods A BR comprising endothelial and epithelial chambers was machined in a biocompatible material. The porcine cornea, securely held, separated the chambers. Medium flow and pressure inside the endothelial chamber were maintained by a peristaltic pump. In the epithelial chamber, the corneal surface was alternatively exposed to air and a specific medium. Two transparent windows allowed thickness measurement by optical coherence tomography without opening the BR. Porcine corneas stored in the BR-on (pressure 20 mm Hg, flow 5 μL/min, temperature 31°C) were compared with (1) BR-off (no pressure or flow); (2) organ culture; and (3) Petri dish with agar on the endothelial side. Epithelial and limbal structure and differentiation, corneal transparency and thickness, and endothelial viability were compared after 7 days of storage and with fresh corneas. Results Corneas stored in the BR-on were thinner and more transparent than those stored with the other methods. The BR-on preserved a stratified and differentiated (K3/K12+) corneal epithelium and undifferentiated basal limbal cells with stemness markers (K3/K12-; ABCB5, K14, p75+), as well as endothelial integrity. Conclusions By recreating equivalent IOP and medium renewal, the BR obtained unprecedented storage quality of porcine corneas and preserved their main epithelial, limbal, and endothelial characteristics.
- Subjects :
- 0301 basic medicine
Intraocular pressure
Endothelium
Cell Survival
Swine
Peristaltic pump
Cell Count
Organ culture
law.invention
Cornea
03 medical and health sciences
Bioreactors
Organ Culture Techniques
0302 clinical medicine
law
medicine
Animals
Corneal epithelium
Chemistry
Petri dish
Cell Differentiation
Equipment Design
Organ Preservation
Anatomy
eye diseases
Epithelium
030104 developmental biology
medicine.anatomical_structure
Models, Animal
030221 ophthalmology & optometry
sense organs
Biomedical engineering
Subjects
Details
- ISSN :
- 15525783
- Volume :
- 58
- Database :
- OpenAIRE
- Journal :
- Investigative Opthalmology & Visual Science
- Accession number :
- edsair.doi.dedup.....9712389ee771023b386aa729487cc62c