Back to Search
Start Over
Endoplasmic-reticulum-mediated microtubule alignment governs cytoplasmic streaming
- Source :
- Nature Cell Biology, Nature Cell Biology, 2017, 19 (4), pp.399-406. ⟨10.1038/ncb3490⟩
- Publication Year :
- 2017
- Publisher :
- HAL CCSD, 2017.
-
Abstract
- Cytoplasmic streaming refers to a collective movement of cytoplasm observed in many cell types. The mechanism of meiotic cytoplasmic streaming (MeiCS) in Caenorhabditis elegans zygotes is puzzling as the direction of the flow is not predefined by cell polarity and occasionally reverses. Here, we demonstrate that the endoplasmic reticulum (ER) network structure is required for the collective flow. Using a combination of RNAi, microscopy and image processing of C. elegans zygotes, we devise a theoretical model, which reproduces and predicts the emergence and reversal of the flow. We propose a positive-feedback mechanism, where a local flow generated along a microtubule is transmitted to neighbouring regions through the ER. This, in turn, aligns microtubules over a broader area to self-organize the collective flow. The proposed model could be applicable to various cytoplasmic streaming phenomena in the absence of predefined polarity. The increased mobility of cortical granules by MeiCS correlates with the efficient exocytosis of the granules to protect the zygotes from osmotic and mechanical stresses.
- Subjects :
- 0301 basic medicine
Zygote
Green Fluorescent Proteins
Cytoplasmic Streaming
Biology
Cytoplasmic Granules
Endoplasmic Reticulum
Microtubules
Time-Lapse Imaging
Xenopus laevis
03 medical and health sciences
0302 clinical medicine
Microtubule
RNA interference
Animals
Caenorhabditis elegans
Cytoskeleton
[PHYS]Physics [physics]
Microscopy, Confocal
Endoplasmic reticulum
Cell Biology
biology.organism_classification
Cytoplasmic streaming
Cell biology
030104 developmental biology
Hydrodynamics
RNA Interference
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Nature Cell Biology, Nature Cell Biology, 2017, 19 (4), pp.399-406. ⟨10.1038/ncb3490⟩
- Accession number :
- edsair.doi.dedup.....97249ca96c08278f2ef50efed530128a