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Studies on the non-invasive anticancer remedy of the triple combination of epigallocatechin gallate, pulsed electric field, and ultrasound
- Source :
- PLoS ONE, Vol 13, Iss 8, p e0201920 (2018), PLoS ONE
- Publication Year :
- 2018
- Publisher :
- Public Library of Science (PLoS), 2018.
-
Abstract
- Cancer is one of the most troublesome diseases and a leading cause of death worldwide. Recently, novel treatments have been continuously developed to improve the disadvantages of conventional therapies, such as prodigious expenses, unwanted side effects, and tumor recurrence. Here, we provide the first non-invasive treatment that has combined epigallocatechin gallate (EGCG), the most abundant catechin in green tea, with a low strength pulsed electric field (PEF) and a low energy ultrasound (US). It has been observed that the cell viability of human pancreatic cancer PANC-1 was decreased approximately to 20% of the control after this combination treatment for 72 h. Besides, the combined triple treatment significantly reduced the high tolerance of HepG2 cells to the EGCG-induced cytotoxicity and similarly exhibited compelling proliferation-inhibitory effects. We also found the combined triple treatment increased the intracellular reactive oxygen species (ROS) and acidic vesicles, and the EGCG-induced inhibition of Akt phosphorylation was dramatically intensified. In this study, the apoptosis inhibitor Z-VAD-FMK and the autophagy inhibitor 3-MA were, respectively, shown to attenuate the anticancer effects of the triple treatment. This indicates that the triple treatment-induced autophagy was switched from cytoprotective to cytotoxic, and hence, cooperatively caused cell death with the apoptosis. Since the EGCG is easily accessible from the green tea and mild for a long-term treatment, and the non-invasive physical stimulations could be modified to focus on a specific location, this combined triple treatment may serve as a promising strategy for anticancer therapy.
- Subjects :
- 0301 basic medicine
Apoptosis Inhibitor
Ultrasonic Therapy
Cancer Treatment
lcsh:Medicine
Apoptosis
Epigallocatechin gallate
Biochemistry
Physical Chemistry
Catechin
chemistry.chemical_compound
Oxidative Damage
0302 clinical medicine
Superoxides
Neoplasms
Medicine and Health Sciences
Enzyme assays
Colorimetric assays
Cytotoxicity
lcsh:Science
Bioassays and physiological analysis
chemistry.chemical_classification
Multidisciplinary
MTT assay
Cell Death
Chemistry
Oxides
Combined Modality Therapy
Oncology
Ultrasonic Waves
Cell Processes
030220 oncology & carcinogenesis
Caspases
Physical Sciences
Cellular Structures and Organelles
Research Article
Anions
Programmed cell death
Cell Survival
Autophagic Cell Death
03 medical and health sciences
Cell Line, Tumor
Autophagy
Humans
Viability assay
Vesicles
Cell Proliferation
Ions
Reactive oxygen species
lcsh:R
Chemical Compounds
Biology and Life Sciences
Cell Biology
Pulsed Radiofrequency Treatment
Research and analysis methods
030104 developmental biology
Biochemical analysis
Cancer research
lcsh:Q
Reactive Oxygen Species
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 13
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....9725ccb1b6c963df984244bab624d263