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Generation of human induced pluripotent stem cell line UNIGEi003-A from skin fibroblasts of an apparently healthy male donor
- Source :
- Stem Cell Research, Vol 48, Iss, Pp 101928-(2020)
- Publication Year :
- 2020
-
Abstract
- Dermal fibroblasts isolated from an apparently healthy 50-year-old man were successfully transformed into induced pluripotent stem cells (iPSCs) by using the integration-free CytoTune-iPS Sendai Reprogramming method. The generated iPSC line has been expanded under feeder-free conditions and displayed all hallmarks of a standard pluripotent stem cell line such as a normal karyotype, expression of pluripotent factors and differentiation capacity into the three germ layers.
- Subjects :
- 0301 basic medicine
Male
Cellular differentiation
Induced Pluripotent Stem Cells
Karyotype
Cell Differentiation
Cell Biology
General Medicine
Germ layer
Biology
Fibroblasts
Middle Aged
Cellular Reprogramming
Cell biology
03 medical and health sciences
030104 developmental biology
0302 clinical medicine
lcsh:Biology (General)
Humans
Induced pluripotent stem cell
Reprogramming
Ipsc line
lcsh:QH301-705.5
030217 neurology & neurosurgery
Developmental Biology
Subjects
Details
- ISSN :
- 18767753
- Volume :
- 48
- Database :
- OpenAIRE
- Journal :
- Stem cell research
- Accession number :
- edsair.doi.dedup.....9751acc8f0d3333595c350135b24f5cf