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Cis-regulatory element-based genome-wide identification of DREB1/CBF targets in Arabidopsis
- Source :
- Progress in Natural Science. 18(5):579-583
- Publication Year :
- 2008
- Publisher :
- Elsevier BV, 2008.
-
Abstract
- Microarray analysis is used to identify transcriptional targets. However, the direct targets of transcription factors cannot be distinguished from indirect ones; further, genes with low-expression levels cannot be identified by this method. In the present study, we exploit the cis -element dehydration-responsive element (DRE) that is known to be responsible for the transcription of DRE binding factor 1 (DREB1) targets in the promoter region of all Arabidopsis genes. Putative targets whose promoters contain the elements were verified by both microarray and reverse transcription-polymerase chain reaction (RT-PCR) analysis. Five new DREB1/CBF direct targets were identified. Compared with traditional microarray analysis, our method is convenient and cost-effective for identifying the downstream targets of transcription factors.
Details
- ISSN :
- 10020071
- Volume :
- 18
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Progress in Natural Science
- Accession number :
- edsair.doi.dedup.....979cf4fe8f5c8248a477e79ba561df20
- Full Text :
- https://doi.org/10.1016/j.pnsc.2007.10.014