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Glial Cell Reaction tocis-Dichlorodiammine Platinum Treatment in the Immature Rat Cerebellum
- Source :
- Experimental Neurology. 144:219-226
- Publication Year :
- 1997
- Publisher :
- Elsevier BV, 1997.
-
Abstract
- In this study we have investigated changes in glial cells of the cerebellum of cis-dichlorodiammine platinum (cisDDP)-treated rats. The expression of S-100 protein and glial fibrillary acidic protein (GFAP), taken as markers of glial cell function, was evaluated using immunocytochemical methods. In parallel, immunoreactivity for calbindin, parvalbumin, and phosphorylated 200-kDa neurofilament protein was observed in Purkinje cells as markers for neuronal integrity and activity. Results showed that, although no difference in the immunostaining of S-100 protein between control and treated animals could be observed, an increase in the frequency of GFAP immunoreactive cells was present in cisDDP-treated rats. In Purkinje cells, immunocytochemical expression of calbindin and parvalbumin was decreased after drug treatment. In addition, following immunoreaction for phosphorylated 200-kDa neurofilament protein, the somata of Purkinje cells, which were negative in control animals, were stained in treated rats. These findings suggest that cisDDP does not significantly interfere with pathways of glial cell activity and that the increased number of GFAP positive astrocytes may be due to an activation of glial cells consequent upon neuronal death.
- Subjects :
- Male
Cerebellum
Neurofilament
Antineoplastic Agents
Calbindin
Purkinje Cells
Developmental Neuroscience
medicine
Animals
Rats, Wistar
biology
Glial fibrillary acidic protein
GFAP stain
Immunohistochemistry
Rats
Cell biology
medicine.anatomical_structure
Animals, Newborn
nervous system
Neurology
biology.protein
Phosphorylation
Cisplatin
Neuroglia
Biomarkers
Parvalbumin
Immunostaining
Subjects
Details
- ISSN :
- 00144886
- Volume :
- 144
- Database :
- OpenAIRE
- Journal :
- Experimental Neurology
- Accession number :
- edsair.doi.dedup.....97df06d1d0aa4f7edcddca56be991e06