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Merging in-solution X-ray and neutron scattering data allows fine structural analysis of membrane-protein detergent complexes
- Source :
- Journal of Physical Chemistry Letters, Journal of Physical Chemistry Letters, American Chemical Society, 2018, 9 (14), pp.3910-3914. ⟨10.1021/acs.jpclett.8b01598⟩, Journal of Physical Chemistry Letters, 2018, 9 (14), pp.3910-3914. ⟨10.1021/acs.jpclett.8b01598⟩
- Publication Year :
- 2018
- Publisher :
- Cold Spring Harbor Laboratory, 2018.
-
Abstract
- International audience; In-solution small-angle X-ray and neutron scattering (SAXS/SANS) have become popular methods to characterize the structure of membrane proteins, solubilized by either detergents or nanodiscs. SANS studies of protein-detergent complexes usually require deuterium-labeled proteins or detergents, which in turn often lead to problems in their expression or purification. Here, we report an approach whose novelty is the combined analysis of SAXS and SANS data from an unlabeled membrane protein complex in solution in two complementary ways. First, an explicit atomic analysis, including both protein and detergent molecules, using the program WAXSiS, which has been adapted to predict SANS data. Second, the use of MONSA which allows one to discriminate between detergent head- and tail-groups in an ab initio approach. Our approach is readily applicable to any detergent-solubilized protein and provides more detailed structural information on protein-detergent complexes from unlabeled samples than SAXS or SANS alone.
- Subjects :
- Materials science
Detergents
Ab initio
Molecular Dynamics Simulation
Neutron scattering
010402 general chemistry
01 natural sciences
Chemistry Techniques, Analytical
Turn (biochemistry)
03 medical and health sciences
X-Ray Diffraction
Molecule
MESH: Molecular Dynamics Simulation
030304 developmental biology
0303 health sciences
[SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM]
Small-angle X-ray scattering
MESH: X-Ray Diffraction
X-ray
Membrane Proteins
MESH: Chemistry Techniques, Analytical
MESH: Neutron Diffraction
0104 chemical sciences
QD450
MESH: Solubility
Neutron Diffraction
Crystallography
Solubility
Membrane protein
Membrane protein complex
MESH: Membrane Proteins
MESH: Detergents
Subjects
Details
- ISSN :
- 19487185
- Database :
- OpenAIRE
- Journal :
- Journal of Physical Chemistry Letters, Journal of Physical Chemistry Letters, American Chemical Society, 2018, 9 (14), pp.3910-3914. ⟨10.1021/acs.jpclett.8b01598⟩, Journal of Physical Chemistry Letters, 2018, 9 (14), pp.3910-3914. ⟨10.1021/acs.jpclett.8b01598⟩
- Accession number :
- edsair.doi.dedup.....97e1a5442ca9e9697fe362bfb92e8f3a