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A Modified SCID Mouse Model of HIV Infection with Utility for Testing Anti-HIV Therapies
- Source :
- AIDS Research and Human Retroviruses. 19:901-908
- Publication Year :
- 2003
- Publisher :
- Mary Ann Liebert Inc, 2003.
-
Abstract
- Using human tumor cells we have developed a mouse model of active HIV infection that may be used for testing antiviral agents, although it does not reflect the pathogenesis of human infection. Irradiated SCID/NOD mice are injected with a tumor of human CD4+ lymphoma cells susceptible to infection and at a separate site, tumor cells persistently infected with either primary or T cell line-adapted strains of HIV. The spread of infection from the infected to the susceptible tumor is monitored as plasma p24 and the presence of HIV-infected cells in the spleen. We have used this model to examine the relative efficacy of neutralizing anti-HIV antibodies to halt the spread of infection. We have found that the tetrameric CD4-antibody fusion protein, CD4-IgG2, is highly effective compared to an anti-V3 loop antibody. This animal model, while not replicating the human disease, allows for the simultaneous testing of efficacy, toxicity, and pharmacokinetics of potential new antiviral therapies. The model can easily be powered to enable comparisons between different therapeutic agents and dosing regimens.
- Subjects :
- Anti-HIV Agents
T cell
Immunology
HIV Infections
Spleen
Mice, SCID
Antibodies, Viral
Virus
Mice
Mice, Inbred NOD
Virology
Tumor Cells, Cultured
medicine
Animals
Humans
NOD mice
biology
medicine.disease
biology.organism_classification
Lymphoma
Disease Models, Animal
Infectious Diseases
medicine.anatomical_structure
Lentivirus
HIV-1
biology.protein
Viral disease
Antibody
CD4 Immunoadhesins
Subjects
Details
- ISSN :
- 19318405 and 08892229
- Volume :
- 19
- Database :
- OpenAIRE
- Journal :
- AIDS Research and Human Retroviruses
- Accession number :
- edsair.doi.dedup.....97fa308f5e3754708abc3a3a99853be6