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Cyclic AMP accumulation in rat soleus muscle: stimulation by beta2- but not beta3-adrenoceptors
- Source :
- European journal of pharmacology. 348(1)
- Publication Year :
- 1998
-
Abstract
- The beta-adrenoceptor subtypes involved in cyclic AMP accumulation in rat soleus muscle were studied using beta1- beta2- and beta3-adrenoceptor agonists and antagonists. Responses to (-)-isoprenaline were antagonised by (-)-propranolol (p KB = 8.32 at 0.1 microM) and by erythro-DL-1(7-methylindian-4-yloxy)-3-isopropylaminobuta n-2-ol (+/-)-ICI 118551) (pKB = 9.38 at 10 nM and 9.65 at 100 nM) but not by 2-hydroxy-5(2-((2-hydroxy-3-(4-((1-methyl-4-trifluoromethyl)1H-imidazole -2-yl)-phenoxy)propyl)amino)ethoxy)-benzamide monomethane sulfonate ((+/-)-CGP 20712A at 10 nM or 100 nM). The beta3-adrenoceptor agonist sodium-4-[-2[-2-hydroxy-2-(-3-chlorophenyl)ethylamino]propyl]phenoxya cetate (BRL 37344 at 10 pM or 10 microM) caused no significant change in basal cyclic AMP levels and had no effect on the level of cyclic AMP accumulation stimulated by (-)-isoprenaline, zinterol or forskolin. (-)-Isoprenaline pretreatment (400 microg kg(-1) h(-1), 14 days) abolished responses to (-)-isoprenaline (10 microM) and zinterol (1 microM) while BRL 37344 had no effect in either isoprenaline or vehicle-treated groups. These results show that beta3-adrenoceptor agonists do not stimulate cyclic AMP accumulation in rat soleus muscle and that (-)-isoprenaline induced increases in cyclic AMP levels are mediated predominantly by beta2-adrenoceptors. This suggests that the previously reported increase in glucose uptake by beta3-adrenoceptor agonists in skeletal muscle does not involve direct stimulation of adenylate cyclase.
- Subjects :
- Agonist
Male
medicine.medical_specialty
medicine.drug_class
Adrenergic beta-Antagonists
Adenylate kinase
Biology
Cyclase
Rats, Sprague-Dawley
chemistry.chemical_compound
Catecholamines
Osmotic Pressure
Internal medicine
Isoprenaline
Receptors, Adrenergic, beta
medicine
Cyclic AMP
Animals
Zinterol
Muscle, Skeletal
Adrenergic beta-2 Receptor Agonists
Pharmacology
Soleus muscle
Forskolin
Isoproterenol
Skeletal muscle
Adrenergic beta-Agonists
Rats
Endocrinology
medicine.anatomical_structure
chemistry
Ethanolamines
Receptors, Adrenergic, beta-3
Indicators and Reagents
medicine.drug
Subjects
Details
- ISSN :
- 00142999
- Volume :
- 348
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- European journal of pharmacology
- Accession number :
- edsair.doi.dedup.....9835d6cfc2f883d50cab9db7f4f79f43