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Insights of Molecular Mechanism of Xylem Development in Five Black Poplar Cultivars
- Source :
- Frontiers in Plant Science, Frontiers in Plant Science, Vol 11 (2020)
- Publication Year :
- 2020
-
Abstract
- Black poplar (Populus deltoides, P. nigra, and their hybrids) is the main poplar cultivars in China. It offers interesting options of large-scale biomass production for bioenergy due to its rapid growth and high yield. Poplar wood properties were associated with chemical components and physical structures during wood formation. In this study, five poplar cultivars, P. euramericana 'Zhonglin46' (Pe1), P. euramericana 'Guariento' (Pe2), P. nigra 'N179' (Pn1), P. deltoides 'Danhong' (Pd1), and P. deltoides 'Nanyang' (Pd2), were used to explore the molecular mechanism of xylem development. We analyzed the structural differences of developing xylem in the five cultivars and profiled the transcriptome-wide gene expression patterns through RNA sequencing. The cross sections of the developing xylem showed that the cell wall thickness of developed fiber in Pd1 was thickest and the number of xylem vessels of Pn1 was the least. A total of 10,331 differentially expressed genes were identified among 10 pairwise comparisons of the five cultivars, most of them were related to programmed cell death and secondary cell wall thickening. K-means cluster analysis and Gene Ontology enrichment analysis showed that the genes highly expressed in Pd1 were related to nucleotide decomposition, metabolic process, transferase, and microtubule cytoskeleton; whereas the genes highly expressed in Pn1 were involved in cell wall macromolecule decomposition and polysaccharide binding processes. Based on a weighted gene co-expression network analysis, a large number of candidate regulators for xylem development were identified. And their potential regulatory roles to cell wall biosynthesis genes were validated by a transient overexpression system. This study provides a set of promising candidate regulators for genetic engineering to improve feedstock and enhance biofuel conversion in the bioenergy crop Populus.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Plant Science
lcsh:Plant culture
01 natural sciences
Black poplar
Cell wall
03 medical and health sciences
Botany
Gene expression
lcsh:SB1-1110
transcriptional regulation
Gene
Hybrid
Original Research
biology
fungi
Xylem
food and beverages
biology.organism_classification
Polysaccharide binding
developing xylem
030104 developmental biology
Populus
cell wall
Secondary cell wall
transcriptome
010606 plant biology & botany
Subjects
Details
- ISSN :
- 1664462X
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- Frontiers in plant science
- Accession number :
- edsair.doi.dedup.....9881016514c815a199208ebef012b69a