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Crystallization and preliminary crystallographic analysis of manganese lipoxygenase
- Source :
- Acta crystallographica. Section F, Structural biology communications. 70(Pt 4)
- Publication Year :
- 2014
-
Abstract
- Lipoxygenases constitute a family of nonhaem metal enzymes with catalytic iron or, occasionally, catalytic manganese. Lipoxygenases oxidize polyunsaturated fatty acids with position specificity and stereospecificity to hydroperoxides, which contribute to inflammation and the development of cancer. Little is known about the structural differences between lipoxygenases with Fe or Mn and the metal-selection mechanism. APichia pastorisexpression system was used for the production of the manganese lipoxygenase of the take-all fungus of wheat,Gaeumannomyces graminis. The active enzyme was treated with α-mannosidase, purified to apparent homogeneity and subjected to crystal screening and X-ray diffraction. The crystals diffracted to 2.6 Å resolution and belonged to space groupC2, with unit-cell parametersa= 226.6,b= 50.6,c= 177.92 Å, β = 91.70°.
- Subjects :
- Stereochemistry
Lipoxygenase
Biophysics
chemistry.chemical_element
Manganese
Crystallography, X-Ray
Biochemistry
Pichia
Catalysis
Pichia pastoris
Stereospecificity
Structural Biology
Genetics
chemistry.chemical_classification
biology
Condensed Matter Physics
biology.organism_classification
Enzyme
chemistry
Crystallization Communications
biological sciences
biology.protein
health occupations
bacteria
Crystallization
Polyunsaturated fatty acid
Subjects
Details
- ISSN :
- 2053230X
- Volume :
- 70
- Issue :
- Pt 4
- Database :
- OpenAIRE
- Journal :
- Acta crystallographica. Section F, Structural biology communications
- Accession number :
- edsair.doi.dedup.....9885cc7de5056deaac42fbbd24d6e7e6