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SIAH1 induced apoptosis by activation of the JNK pathway and inhibited invasion by inactivation of the ERK pathway in breast cancer cells
- Source :
- Cancer Science. 101:73-79
- Publication Year :
- 2010
- Publisher :
- Wiley, 2010.
-
Abstract
- Seven in absentia homolog 1 (SIAH1), a homologue of Drosophila seven in absentia (Sina), has emerged as a tumor suppressor and plays an important role in regulating cell apoptosis. To investigate the role and possible mechanism of SIAH1 in breast cancer cells, we up-regulated the expression of SIAH1 using pcDNA3-myc-SIAH1 and knocked down SIAH1 using SIAH1 siRNA. We found that the overexpression of SIAH1 induced cell apoptosis by up-regulating the level of Bim through the activation of the JNK signaling pathway, and the suppression of SIAH1 expression increased cell invasion via the activation of the ERK signaling pathway in breast cancer cells. All these results indicate that the JNK and ERK signaling pathways may play an important role in the SIAH1-dependent biological behavior of breast cancer, and it may be a good molecular therapeutic target to increase the expression level of SIAH1 through promoting cell apoptosis and inhibiting cell invasion in human breast cancer.
- Subjects :
- MAPK/ERK pathway
Cancer Research
Programmed cell death
MAP Kinase Signaling System
Ubiquitin-Protein Ligases
Apoptosis
Breast Neoplasms
SIAH1
Biology
Metastasis
Cell Line, Tumor
Proto-Oncogene Proteins
medicine
Humans
Neoplasm Invasiveness
Extracellular Signal-Regulated MAP Kinases
Bcl-2-Like Protein 11
JNK Mitogen-Activated Protein Kinases
Membrane Proteins
Nuclear Proteins
Cancer
General Medicine
medicine.disease
Oncology
Mitogen-activated protein kinase
Cancer research
biology.protein
Female
Breast disease
Apoptosis Regulatory Proteins
Subjects
Details
- ISSN :
- 13497006 and 13479032
- Volume :
- 101
- Database :
- OpenAIRE
- Journal :
- Cancer Science
- Accession number :
- edsair.doi.dedup.....98a0d18b20e922b7b30ff45bc08b3659