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Cell Density-Dependent Regulation of Hepatic Development by a gp130-Independent Pathway
- Source :
- Biochemical and Biophysical Research Communications. 277:152-158
- Publication Year :
- 2000
- Publisher :
- Elsevier BV, 2000.
-
Abstract
- We previously demonstrated that oncostatin M (OSM) promotes hepatic development in concert with glucocorticoid. The livers from mice deficient for gp130, a signaling subunit of the OSM receptor, displayed reduced expression of hepatic differentiation marker and defective glycogenic function. However, these phenotypes were not completely abolished in gp130(-/-) mice, suggesting that there is an alternative pathway regulating hepatic development in vivo. To test this possibility, we cultured gp130(-/-) fetal hepatic cells and investigated a signal that induces hepatic differentiation. When hepatocytes were forced to interact with each other by inoculating cells at high densities, hepatic differentiation was induced even in the absence of gp130. Moreover, cells stimulated with OSM and/or cultured at a high density possess many other metabolic functions. These observations suggest that fetal hepatic cells acquire multiple characteristics of differentiated hepatocytes in response to the signals generated by cell-cell contacts as well as by OSM.
- Subjects :
- STAT3 Transcription Factor
medicine.medical_specialty
Cell signaling
Liver cytology
Cellular differentiation
Biophysics
Cell Count
Cell Communication
Oncostatin M
Biochemistry
Mice
Ammonia
Antigens, CD
Internal medicine
Cytokine Receptor gp130
medicine
Animals
RNA, Messenger
Phosphorylation
Receptors, Cytokine
Receptor
STAT3
Molecular Biology
Cells, Cultured
Serum Albumin
Mice, Knockout
Membrane Glycoproteins
biology
Histocytochemistry
fungi
Gene Expression Regulation, Developmental
Cell Differentiation
Receptors, Oncostatin M
Cell Biology
Glycoprotein 130
Antigens, Differentiation
Liver Glycogen
Cell biology
DNA-Binding Proteins
Endocrinology
Liver
Hepatocytes
Trans-Activators
biology.protein
Hepatic stellate cell
Peptides
Gene Deletion
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 277
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....98d56886a53cc11ef39b8c33596b7515