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Nioella nitratireducens gen. nov., sp. nov., a novel member of the family Rhodobacteraceae isolated from Azorean Island
- Source :
- Antonie van Leeuwenhoek. 107:589-595
- Publication Year :
- 2014
- Publisher :
- Springer Science and Business Media LLC, 2014.
-
Abstract
- A novel Gram-negative, non-spore forming, rod-shaped aerobic bacterium, designated SSW136(T), was isolated from a surface seawater sample collected at Espalamaca (in Faial Island), Azores. Growth was found to occur from 10 to 37 °C, pH 6.0-8.0, and with 2-11 % of NaCl. 16S rRNA gene sequence indicated that the strain SSW136(T) belongs to the family Rhodobacteraceae. Strain SSW136(T) exhibited 96.3, 95.9, 95.7 and 95.5 sequence similarity to the type strains Oceanicola litoreus M-M22(T), Roseovarius aestuarii SMK-122(T), Marivita geojedonensis DPG-138(T), and Pseudoruegeria aquimaris SW-255(T) respectively. Neighbour-joining and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences revealed that strain SSW136(T) was affiliated to the family Rhodobacteraceae and formed a separate branch. The G+C content was 63.5 mol%. The major respiratory quinone was found to be Q-10. The polar lipids of strain SSW136(T) consisted of phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, two unidentified aminolipids and three unidentified phospholipids. The major fatty acids were C18:1 ω7c (46.5 %), Cyclo-C19:0 ω8c (16.0 %) and C16:0 (12.8 %). On the basis of the morphological, genotypic, chemotaxonomic characteristics and low DNA-DNA relatedness, strain SSW136(T) is proposed to represent a novel genus and novel species, Nioella nitratireducens gen. nov., sp. nov., in the family Rhodobacteraceae. The type strain is SSW136(T) (=KCTC 32417(T) = NCIM 5499(T)).
- Subjects :
- DNA, Bacterial
Sequence analysis
Aerobic bacteria
Molecular Sequence Data
Sodium Chloride
Biology
DNA, Ribosomal
Microbiology
Cytosol
Genus
Phylogenetics
RNA, Ribosomal, 16S
Cluster Analysis
Seawater
Rhodobacteraceae
Molecular Biology
Azores
Phospholipids
Phylogeny
Base Composition
Strain (chemistry)
Phylogenetic tree
Fatty Acids
Quinones
Temperature
Nucleic Acid Hybridization
Sequence Analysis, DNA
General Medicine
Hydrogen-Ion Concentration
Ribosomal RNA
16S ribosomal RNA
Aerobiosis
Subjects
Details
- ISSN :
- 15729699 and 00036072
- Volume :
- 107
- Database :
- OpenAIRE
- Journal :
- Antonie van Leeuwenhoek
- Accession number :
- edsair.doi.dedup.....98d9f650f48410510e2224975729707c