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Glutathione‐dependent denitrosation of GSNOR1 promotes oxidative signalling downstream of H 2 O 2
- Source :
- Plant, Cell and Environment, Plant, Cell and Environment, 2020, 43 (5), pp.1175-1191. ⟨10.1111/pce.13727⟩, Plant, Cell and Environment, Wiley, 2020, 43 (5), pp.1175-1191. ⟨10.1111/pce.13727⟩
- Publication Year :
- 2020
- Publisher :
- HAL CCSD, 2020.
-
Abstract
- Photorespiratory hydrogen peroxide (H2 O2 ) plays key roles in pathogenesis responses by triggering the salicylic acid (SA) pathway in Arabidopsis. However, factors linking intracellular H2 O2 to activation of the SA pathway remain elusive. In this work, the catalase-deficient Arabidopsis mutant, cat2, was exploited to elucidate the impact of S-nitrosoglutathione reductase 1 (GSNOR1) on H2 O2 -dependent signalling pathways. Introducing the gsnor1-3 mutation into the cat2 background increased S-nitrosothiol levels and abolished cat2-triggered cell death, SA accumulation, and associated gene expression but had little additional effect on the major components of the ascorbate-glutathione system or glycolate oxidase activities. Differential transcriptome profiles between gsnor1-3 and cat2 gsnor1-3 together with damped ROS-triggered gene expression in cat2 gsnor1-3 further indicated that GSNOR1 acts to mediate the SA pathway downstream of H2 O2 . Up-regulation of GSNOR activity was compromised in cat2 cad2 and cat2 pad2 mutants in which glutathione accumulation was genetically prevented. Experiments with purified recombinant GSNOR revealed that the enzyme is posttranslationally regulated by direct denitrosation in a glutathione-dependent manner. Together, our findings identify GSNOR1-controlled nitrosation as a key factor in activation of the SA pathway by H2 O2 and reveal that glutathione is required to maintain this biological function.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Programmed cell death
Physiology
H2O2
salicylic acid
[SDV]Life Sciences [q-bio]
Mutant
Plant Science
Oxidative phosphorylation
Reductase
01 natural sciences
03 medical and health sciences
chemistry.chemical_compound
Arabidopsis
Gene expression
oxidative stress
glutathione
denitrosation
GSNOR1
biology
Glutathione
biology.organism_classification
Cell biology
030104 developmental biology
chemistry
Intracellular
010606 plant biology & botany
Subjects
Details
- Language :
- English
- ISSN :
- 01407791 and 13653040
- Database :
- OpenAIRE
- Journal :
- Plant, Cell and Environment, Plant, Cell and Environment, 2020, 43 (5), pp.1175-1191. ⟨10.1111/pce.13727⟩, Plant, Cell and Environment, Wiley, 2020, 43 (5), pp.1175-1191. ⟨10.1111/pce.13727⟩
- Accession number :
- edsair.doi.dedup.....98db9579222d7a6680d6a026b03d6cd6