Back to Search
Start Over
The Comparative Sensitivity of Immunohistochemical Markers of Megakaryocytic Differentiation in Acute Megakaryoblastic Leukemia
- Source :
- American Journal of Clinical Pathology. 150:461-467
- Publication Year :
- 2018
- Publisher :
- Oxford University Press (OUP), 2018.
-
Abstract
- Objectives Immunohistochemistry (IHC) staining of core biopsy sections often plays an essential role in the diagnosis of acute megakaryoblastic leukemia (AMKL). The goal of this study was to define the relative sensitivities of commonly used stains for markers of megakaryocytic differentiation. Methods The sensitivities of IHC stains for CD42b, CD61, and von Willebrand factor (vWF) were compared in 32 cases of pediatric AMKL. Results The sensitivities of CD42b, CD61, and vWF were 90.6%, 78.1% and 62.5%, respectively. When CD42b and CD61 were used together, the combined sensitivity increased to 93.6%. There were no cases in which vWF was positive when both CD42b and CD61 were negative. Conclusions CD42b can reliably be used as a solitary first-line marker for blasts of megakaryocytic lineage, whereas CD61 may be reserved for infrequent cases that are CD42b negative. There is no role for the routine use of vWF when CD42b and CD61 are available.
- Subjects :
- 0301 basic medicine
Pathology
medicine.medical_specialty
Cellular differentiation
Sensitivity and Specificity
03 medical and health sciences
Acute megakaryoblastic leukemia
0302 clinical medicine
Von Willebrand factor
Bone Marrow
Leukemia, Megakaryoblastic, Acute
hemic and lymphatic diseases
von Willebrand Factor
Biopsy
medicine
Humans
Child
biology
medicine.diagnostic_test
business.industry
Integrin beta3
Cell Differentiation
General Medicine
medicine.disease
Immunohistochemistry
Staining
Leukemia
030104 developmental biology
Platelet Glycoprotein GPIb-IX Complex
030220 oncology & carcinogenesis
biology.protein
Biopsy, Large-Core Needle
Reagent Kits, Diagnostic
business
Megakaryocytes
CD61
Biomarkers
Subjects
Details
- ISSN :
- 19437722 and 00029173
- Volume :
- 150
- Database :
- OpenAIRE
- Journal :
- American Journal of Clinical Pathology
- Accession number :
- edsair.doi.dedup.....98ec53851174704b896b1328a74d1c97