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LFA1 and ICAM1 are critical for fusion and spread of murine leukemia virus in vivo
- Source :
- Cell reports. 38(3)
- Publication Year :
- 2021
-
Abstract
- Murine leukemia virus (MLV)-presenting cells form stable intercellular contacts with target cells during infection of lymphoid tissue, indicating a role of cell-cell contacts in retrovirus dissemination. Whether host cell adhesion proteins are required for retrovirus spread in vivo remains unknown. Here, we demonstrate that the lymphocyte-function-associated-antigen-1 (LFA1) and its ligand intercellular-adhesion-molecule-1 (ICAM1) are important for cell-contact-dependent transmission of MLV between leukocytes. Infection experiments in LFA1- and ICAM1-deficient mice demonstrate a defect in MLV spread within lymph nodes. Co-culture of primary leukocytes reveals a specific requirement for ICAM1 on donor cells and LFA1 on target cells for cell-contact-dependent spread through trans- and cis-infection. Importantly, adoptive transfer experiments combined with a newly established MLV-fusion assay confirm that the directed LFA1-ICAM1 interaction is important for retrovirus fusion and transmission in vivo. Taken together, our data provide insights on how retroviruses exploit host proteins and the biology of cell-cell interactions for dissemination.
- Subjects :
- Leukemia Virus, Murine
Mice, Inbred C57BL
Mice
Tumor Virus Infections
Leukemia, Experimental
Macrophages
Host-Pathogen Interactions
Animals
Lymphocytes
Intercellular Adhesion Molecule-1
General Biochemistry, Genetics and Molecular Biology
Lymphocyte Function-Associated Antigen-1
Retroviridae Infections
Subjects
Details
- ISSN :
- 22111247
- Volume :
- 38
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Cell reports
- Accession number :
- edsair.doi.dedup.....994e304f9c8e0c9ca149a4785d9ab82c