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Inhibition of maternally expressed gene 3 attenuated lipopolysaccharide‐induced apoptosis through sponging miR‐21 in renal tubular epithelial cells
- Source :
- Journal of Cellular Biochemistry. 119:7800-7806
- Publication Year :
- 2018
- Publisher :
- Wiley, 2018.
-
Abstract
- Acute kidney injury (AKI) results in retention of waste products and dysregulation of extracellular volume and electrolytes, thus leading to a variety of complications. Recent advances in long noncoding RNAs suggested their close relationship with disease progression. In the current study, we investigated the role and mechanism of maternally expressed gene 3 (MEG3) on AKI pathogenesis. Real-time polymerase chain reaction found that the expression of MEG3 was significantly increased in both kidney tissues and TKPTS cells induced by lipopolysaccharide (LPS). Western blot assay showed that the expression of apoptosis regulator Bcl-2 was increased in MEG3-inhibited TKPTS cells. Flow cytometry assay confirmed that LPS-induced apoptosis was significantly attenuated after transfection of si-MEG3. The RNAhybrid informatics algorithm predicted that there was a strong binding capacity between miR-21 and MEG3. Luciferase reporter assay confirmed that MEG3 could function as a competing endogenous RNA of miR-21. The antiapoptotic effect of si-MEG3 could be neutralized by a miR-21 inhibitor, demonstrated by the decreased expression of Bcl-2 and flow cytometry results. Further investigation showed that programmed cell death protein 4 (PDCD4), a validated target of miR-21, was highly expressed in both injured kidney tissues and LPS-stimulated TKPTS cells. Meanwhile, the protein expression of PDCD4 was significantly reduced by inhibition of MEG3, but retrieved by coinhibition of MEG3 and miR-21. In conclusion, our results demonstrated that inhibition of MEG3 could attenuate LPS-induced apoptosis in TKPTS cells by regulating the miR-21/PDCD4 pathway, suggesting that the MEG3/miR-21/PDCD4 axis could be developed as a potential therapeutic target of AKI.
- Subjects :
- Lipopolysaccharides
0301 basic medicine
Apoptosis
Biochemistry
Cell Line
Flow cytometry
Mice
03 medical and health sciences
0302 clinical medicine
Gene expression
medicine
Animals
Programmed Cell Death Protein 4
RNA, Small Interfering
3' Untranslated Regions
Molecular Biology
MEG3
Kidney
medicine.diagnostic_test
Chemistry
Apoptosis Regulator
RNA-Binding Proteins
Cell Biology
Transfection
Acute Kidney Injury
Molecular biology
Up-Regulation
Disease Models, Animal
MicroRNAs
Kidney Tubules
030104 developmental biology
medicine.anatomical_structure
Proto-Oncogene Proteins c-bcl-2
030220 oncology & carcinogenesis
RNA, Long Noncoding
Apoptosis Regulatory Proteins
Algorithms
Subjects
Details
- ISSN :
- 10974644 and 07302312
- Volume :
- 119
- Database :
- OpenAIRE
- Journal :
- Journal of Cellular Biochemistry
- Accession number :
- edsair.doi.dedup.....997ed47a2a94b936f01ccd7aac382129