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A Large-Scale Screen for Mutagen-Sensitive Loci in Drosophila
- Source :
- Genetics, Genetics, Genetics Society of America, 2004, 167 (1), pp.217-231. ⟨10.1534/genetics.167.1.217⟩
- Publication Year :
- 2004
- Publisher :
- Oxford University Press (OUP), 2004.
-
Abstract
- In a screen for new DNA repair mutants, we tested 6275 Drosophila strains bearing homozygous mutagenized autosomes (obtained from C. Zuker) for hypersensitivity to methyl methanesulfonate (MMS) and nitrogen mustard (HN2). Testing of 2585 second-chromosome lines resulted in the recovery of 18 mutants, 8 of which were alleles of known genes. The remaining 10 second-chromosome mutants were solely sensitive to MMS and define 8 new mutagen-sensitive genes (mus212–mus219). Testing of 3690 third chromosomes led to the identification of 60 third-chromosome mutants, 44 of which were alleles of known genes. The remaining 16 mutants define 14 new mutagen-sensitive genes (mus314–mus327). We have initiated efforts to identify these genes at the molecular level and report here the first two identified. The HN2-sensitive mus322 mutant defines the Drosophila ortholog of the yeast snm1 gene, and the MMS- and HN2-sensitive mus301 mutant defines the Drosophila ortholog of the human HEL308 gene. We have also identified a second-chromosome mutant, mus215ZIII-2059, that uniformly reduces the frequency of meiotic recombination to
- Subjects :
- DNA Repair
MESH: Sequence Homology, Amino Acid
[SDV]Life Sciences [q-bio]
Mutant
MESH: Methyl Methanesulfonate
MESH: Amino Acid Sequence
MESH: Base Sequence
medicine.disease_cause
chemistry.chemical_compound
0302 clinical medicine
Nondisjunction, Genetic
MESH: Animals
Recombination, Genetic
MESH: DNA Repair
Genetics
MESH: Genetic Complementation Test
0303 health sciences
Mutation
MESH: Mechlorethamine
biology
MESH: DNA
Chromosome Mapping
MESH: Nondisjunction, Genetic
MESH: Saccharomyces cerevisiae
3. Good health
Meiosis
Drosophila melanogaster
Genetic Techniques
MESH: Recombination, Genetic
Research Article
MESH: Mutation
DNA repair
Molecular Sequence Data
Saccharomyces cerevisiae
MESH: Drosophila melanogaster
03 medical and health sciences
MESH: Mutagens
medicine
Animals
Amino Acid Sequence
Mechlorethamine
Gene
Alleles
030304 developmental biology
MESH: Molecular Sequence Data
Base Sequence
Sequence Homology, Amino Acid
MESH: Alleles
Genetic Complementation Test
fungi
DNA
Methyl Methanesulfonate
biology.organism_classification
Molecular biology
Methyl methanesulfonate
MESH: Meiosis
MESH: Genetic Techniques
chemistry
MESH: Chromosome Mapping
Homologous recombination
030217 neurology & neurosurgery
Mutagens
Subjects
Details
- ISSN :
- 19432631 and 00166731
- Volume :
- 167
- Database :
- OpenAIRE
- Journal :
- Genetics
- Accession number :
- edsair.doi.dedup.....99a2fbad5ee5a4b7cf52976730c0d216