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Cucurbitacin I induces apoptosis in ovarian cancer cells through oxidative stress and the p190B‑Rac1 signaling axis
- Source :
- Molecular Medicine Reports. 22:2545-2550
- Publication Year :
- 2020
- Publisher :
- Spandidos Publications, 2020.
-
Abstract
- Ovarian cancer is a serious threat to women's life and health, with a high mortality rate. Therefore, in addition to improving surgery for ovarian cancer, it is particularly important to develop novel drug treatments. In the present study, the anticancer effects of cucurbitacin I, a natural product, were investigated. Cucurbitacin I impaired the viability of SKVO3 cells in a concentration‑ and time‑dependent manner. Apoptosis was involved in the process of cucurbitacin I‑induced cell death, with an increase observed in cleaved‑caspase 3 and BAX, and a decrease in Bcl‑2. Cucurbitacin I caused a notable increase in intracellular reactive oxygen species, and regulated Kelch‑like ECH‑associated protein 1 and nuclear factor erythroid‑derived 2‑like 2 to decrease the expression of antioxidant‑related genes. In addition, Cucurbitacin I induced cell shrinkage by regulating the p190BRhoGAP (p190B)‑Rac1 signaling axis related to the cytoskeleton. In brief, these results suggested that cucurbitacin I induced cell death through oxidative stress and the p190B‑Rac1 signaling axis in SKVO3 cells. The results may provide novel evidence for the treatment of ovarian cancer.
- Subjects :
- rac1 GTP-Binding Protein
0301 basic medicine
Cancer Research
Programmed cell death
Time Factors
Cell Survival
Cell
medicine.disease_cause
Biochemistry
03 medical and health sciences
0302 clinical medicine
Cell Line, Tumor
Genetics
medicine
Humans
Molecular Biology
Ovarian Neoplasms
Oncogene
Cucurbitacin
Chemistry
GTPase-Activating Proteins
Cell cycle
medicine.disease
Triterpenes
Gene Expression Regulation, Neoplastic
Oxidative Stress
030104 developmental biology
medicine.anatomical_structure
Oncology
Apoptosis
030220 oncology & carcinogenesis
Cancer research
Molecular Medicine
Female
Ovarian cancer
Oxidative stress
Signal Transduction
Subjects
Details
- ISSN :
- 17913004 and 17912997
- Volume :
- 22
- Database :
- OpenAIRE
- Journal :
- Molecular Medicine Reports
- Accession number :
- edsair.doi.dedup.....99bf6e151eed973493aa5e7310f22fba