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A Multiperspective Approach to Solvent Regulation of Enzymatic Activity : HMG-CoA Reductase
- Publication Year :
- 2018
-
Abstract
- 3-Hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase was investigated in different organic cosolvents by means of kinetic and calorimetric measurements, molecular dynamics simulations, and small-angle X-ray scattering. The combined experimental and theoretical techniques were essential to complement each other's limitations in the investigation of the complex interaction pattern between the enzyme, different solvent types, and concentrations. In this way, the underlying mechanisms for the loss of enzyme activity in different water-miscible solvents could be elucidated. These include direct inhibitory effects onto the active center and structural distortions.
- Subjects :
- 0301 basic medicine
Acetonitriles
Molecular model
Chemie
Ionic Liquids
Calorimetry
Molecular Dynamics Simulation
Reductase
010402 general chemistry
01 natural sciences
Biochemistry
Active center
03 medical and health sciences
Molecular dynamics
X-Ray Diffraction
Scattering, Small Angle
Molecular Biology
chemistry.chemical_classification
biology
Organic Chemistry
Enzyme assay
0104 chemical sciences
Solvent
Kinetics
030104 developmental biology
Enzyme
chemistry
Alcohols
Solvents
Sulfolobus solfataricus
Biophysics
biology.protein
Molecular Medicine
Acyl Coenzyme A
Solvent effects
Biologie
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....99c8e0657527afb7542241e72b3e1c4f