Supplemental Figure 3 from Herbacetin Is a Novel Allosteric Inhibitor of Ornithine Decarboxylase with Antitumor Activity

The effect of polyamines on intracellular herbacetin concentration. The effect of polyamines on the intracellular herbacetin level was determined using HCT116 colon cancer cells. Cells were treated with herbacetin and putrescine, spermidine or spermine for 5 h in medium supplemented with 10% FBS and intracellular herbacetin was extracted and analyzed by using HPLC. All data are represented as means {plus minus} S.D. of triplicate values from 3 independent experiments.