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Screening of differentially expressed immune-related genes from spleen of broilers fed with probiotic Bacillus cereus PAS38 based on suppression subtractive hybridization
- Source :
- PLoS ONE, PLoS ONE, Vol 14, Iss 12, p e0226829 (2019)
- Publication Year :
- 2019
- Publisher :
- Public Library of Science (PLoS), 2019.
-
Abstract
- The aim of this study was to construct the spleen differential genes library of broilers fed with probiotic Bacillus cereus PAS38 by suppression subtractive hybridization (SSH) and screen the immune-related genes. Sixty seven-day-old broilers were randomly divided into two groups. The control group was fed with basal diet, and the treated group was fed with basal diet containing Bacillus cereus PAS38 1×106 CFU/g. Spleen tissues were taken and extracted its total RNA at 42 days old, then SSH was used to construct differential gene library and screen immune-related genes. A total of 119 differentially expressed sequence tags (ESTs) were isolated by SSH and 9 immune-related genes were screened out by Gene ontology analysis. Nine differentially expressed genes were identified by qRT-PCR. JCHAIN, FTH1, P2RX7, TLR7, IGF1R, SMAD7, and SLC7A6 were found to be significantly up-regulated in the treated group. Which was consistent with the results of SSH. These findings imply that probiotic Bacillus cereus PAS38-induced differentially expressed genes in spleen might play an important role in the improvement of immunity for broilers, which provided useful information for further understanding of the molecular mechanism of probiotics responsible to affect the poultry immunity.
- Subjects :
- Male
0301 basic medicine
Genetic Screens
Physiology
Gene Identification and Analysis
Bacillus cereus
Gene Expression
Bacillus
Artificial Gene Amplification and Extension
Pathology and Laboratory Medicine
Polymerase Chain Reaction
Immune Receptors
Biochemistry
Poultry
Transcriptome
0302 clinical medicine
Immune Physiology
Gene expression
Medicine and Health Sciences
Gamefowl
Toll-like Receptors
Bacillus Cereus
Expressed sequence tag
Immune System Proteins
Multidisciplinary
Eukaryota
Bacterial Pathogens
Medical Microbiology
030220 oncology & carcinogenesis
Vertebrates
Medicine
Female
Pathogens
Research Article
Signal Transduction
Subtractive Hybridization Techniques
Science
Immunology
Biology
Research and Analysis Methods
Microbiology
Birds
03 medical and health sciences
Genetics
Animals
Molecular Biology Techniques
Microbial Pathogens
Molecular Biology
Gene
Bacteria
Probiotics
Organisms
Biology and Life Sciences
Proteins
Cell Biology
biology.organism_classification
Animal Feed
030104 developmental biology
Gene Expression Regulation
Fowl
Suppression subtractive hybridization
Amniotes
bacteria
Chickens
Spleen
Genetic screen
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 14
- Database :
- OpenAIRE
- Journal :
- PLOS ONE
- Accession number :
- edsair.doi.dedup.....9a4a34c1dffceb115b24bb53c58627dc