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Levels of F2-isoprostanes, F4-neuroprostanes, and total nitrate/nitrite in plasma and cerebrospinal fluid of patients with traumatic brain injury
- Source :
- Free Radical Research. 49:1419-1430
- Publication Year :
- 2015
- Publisher :
- Informa UK Limited, 2015.
-
Abstract
- Several events occurring during the secondary damage of traumatic brain injury (TBI) can cause oxidative stress. F(2)-isoprostanes (F(2)-IsoPs) and F(4)-neuroprostanes (F(4)-NPs) are specific lipid peroxidation markers generated from arachidonic acid and docosahexaenoic acid, respectively. In this study, we evaluated oxidative stress in patients with moderate and severe TBI. Since sedatives are routinely used to treat TBI patients and propofol has been considered an antioxidant, TBI patients were randomly treated with propofol or midazolam for 72 h postoperation. We postoperatively collected cerebrospinal fluid (CSF) and plasma from 15 TBI patients for 6-10 d and a single specimen of CSF or plasma from 11 controls. Compared with the controls, the TBI patients exhibited elevated levels of F(2)-IsoPs and F(4)-NPs in CSF throughout the postsurgery period regardless of the sedative used. Compared with the group of patients who received midazolam, those who received propofol exhibited markedly augmented levels of plasma F(2)-IsoPs, which were associated with higher F(4)-NPs levels and lower total nitrate/nitrite levels in CSF early in the postsurgery period. Furthermore, the higher CSF F(2)-IsoPs levels correlated with 6-month and 12-month worse outcomes, which were graded according to the Glasgow Outcome Scale. The results demonstrate enhanced oxidative damage in the brain of TBI patients and the association of higher CSF levels of F(2)-IsoPs with a poor outcome. Moreover, propofol treatment might promote lipid peroxidation in the circulation, despite possibly suppressing nitric oxide or peroxynitrite levels in CSF, because of the increased loading of the lipid components from the propofol infusion.
- Subjects :
- Adult
Male
Chromatography, Gas
Adolescent
Traumatic brain injury
Midazolam
Enzyme-Linked Immunosorbent Assay
medicine.disease_cause
Biochemistry
Mass Spectrometry
Nitric oxide
Lipid peroxidation
Young Adult
chemistry.chemical_compound
Cerebrospinal fluid
medicine
Humans
Neuroprostanes
Glasgow Coma Scale
Propofol
Nitrites
Aged
F2-Isoprostanes
Nitrates
Glasgow Outcome Scale
General Medicine
Middle Aged
medicine.disease
Oxidative Stress
chemistry
Brain Injuries
Anesthesia
Female
Lipid Peroxidation
Anesthetics, Intravenous
Biomarkers
Peroxynitrite
Oxidative stress
medicine.drug
Subjects
Details
- ISSN :
- 10292470 and 10715762
- Volume :
- 49
- Database :
- OpenAIRE
- Journal :
- Free Radical Research
- Accession number :
- edsair.doi.dedup.....9a780c801279888156a2c7bcc671d458