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Characterization of upper airway ciliary beat by coupling isolated and collective cilia motion analysis

Authors :
Louis
Daniel Isabey
Mathieu Bottier
Estelle Escudier
Sylvain Blanchon
Marcel Filoche
André Coste
Jean-François Papon
Source :
Cilia
Publication Year :
2015
Publisher :
BioMed Central, 2015.

Abstract

Methods Ciliated cells issued from nasal brushing (controls and primary ciliary dyskinesia patients) were recorded by high-speed video-microscopy (350 frames s). We have performed an original quantitative analysis of ciliary beat dynamics (CBD) by following cilium tips. It allows to describe different parameters including ciliary beat frequency also measured by Fast-Fourier-Transform and Video-Kymography. We have also developed the microbeads tracking method (MBT) to get an index of the global efficiency of ciliary beat. Here, micro-beads (4.5 μm) have been used as markers of the flow generated by beating cilia.

Details

Language :
English
ISSN :
20462530
Volume :
4
Issue :
Suppl 1
Database :
OpenAIRE
Journal :
Cilia
Accession number :
edsair.doi.dedup.....9a822dc1da8601651a821a040550e81c