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Silk scaffolds with gradient pore structure and improved cell infiltration performance
- Source :
- Materials scienceengineering. C, Materials for biological applications. 94
- Publication Year :
- 2018
-
Abstract
- Electrospun scaffold with three-dimensional (3D) geometry and appropriate pore structure is an important challenge to mimic natural tissues such as skin, cartilage, etc. In this work, 3D silk fibroin (SF) electrospun scaffolds with gradient pore size were prepared by combining multi-step electrospinning with low temperature (LTE) collecting. The LTE electrospun scaffolds achieved 3D macro-structure with large pore size. The effects of relative humidity (RH), collecting temperature on the morphology of the scaffolds were investigated by scanning electron microscopy and computed tomography. The pore size of the scaffolds was tailored by adjusting SF concentration, electric field, flow rate, needle gauge and collector temperature during electrospinning at 50% RH. L929 cell infiltration results of the scaffolds showed that conventional electrospun scaffolds with small pore size (average diameter 5.9 ± 1.4 μm) restrained cell proliferation and infiltration. On the contrary, LTE electrospun scaffolds with medium pore size (average diameter 11.6 ± 1.4 μm) improved cell proliferation obviously. Large pore size scaffolds (average diameter 37.2 ± 12.9 μm) was beneficial to cell infiltration depth in the thickness direction of the scaffolds. The scaffolds, which were integrated with layers of small, medium and large pores, are promising in the repair of tissue with gradient pore structures.
- Subjects :
- Pore size
Scaffold
Materials science
Scanning electron microscope
Silk
Fibroin
Bioengineering
02 engineering and technology
010402 general chemistry
01 natural sciences
Biomaterials
Mice
medicine
Animals
Cell Shape
Cell Proliferation
Tissue Scaffolds
Humidity
Fibroblasts
021001 nanoscience & nanotechnology
medicine.disease
Bombyx
Electrospinning
0104 chemical sciences
Volumetric flow rate
SILK
Mechanics of Materials
NIH 3T3 Cells
0210 nano-technology
Infiltration (medical)
Porosity
Biomedical engineering
Subjects
Details
- ISSN :
- 18730191
- Volume :
- 94
- Database :
- OpenAIRE
- Journal :
- Materials scienceengineering. C, Materials for biological applications
- Accession number :
- edsair.doi.dedup.....9a9b801d9cd50c65b9a3b768cb520cd4