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α-Fucosidases with different substrate specificities from two species of Fusarium
- Source :
- Applied Microbiology and Biotechnology. 97:5371-5380
- Publication Year :
- 2012
- Publisher :
- Springer Science and Business Media LLC, 2012.
-
Abstract
- Two fungal-secreted α-fucosidases and their genes were characterized. FoFCO1 was purified from culture filtrates of Fusarium oxysporum strain 0685 grown on l-fucose and its encoding gene identified in the sequenced genome of strain 4287. FoFCO1 was active on p-nitrophenyl-α-fucoside (pNP-Fuc), but did not defucosylate a nonasaccharide (XXFG) fragment of pea xyloglucan. A putative α-fucosidase gene (FgFCO1) from Fusarium graminearum was expressed in Pichia pastoris. FgFCO1 was ∼1,800 times less active on pNP-Fuc than FoFCO1, but was able to defucosylate the XXFG nonasaccharide. Although FgFCO1 and FoFCO1 both belong to Glycosyl Hydrolase family 29, they share
- Subjects :
- alpha-L-Fucosidase
Genetics
Subfamily
Sequence Homology, Amino Acid
Sequence analysis
Molecular Sequence Data
Fungal genetics
food and beverages
Sequence alignment
Sequence Analysis, DNA
General Medicine
Biology
biology.organism_classification
Applied Microbiology and Biotechnology
Substrate Specificity
Pichia pastoris
Microbiology
Fusarium
Fusarium oxysporum
Glycosides
DNA, Fungal
Sequence Alignment
Gene
Trichoderma reesei
Biotechnology
Subjects
Details
- ISSN :
- 14320614 and 01757598
- Volume :
- 97
- Database :
- OpenAIRE
- Journal :
- Applied Microbiology and Biotechnology
- Accession number :
- edsair.doi.dedup.....9aa7bf219aa773f59edbf828967c8000