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Modeling Myotonic Dystrophy 1 in C2C12 Myoblast Cells
- Source :
- Journal of visualized experiments : JoVE. (113)
- Publication Year :
- 2016
-
Abstract
- Myotonic dystrophy 1 (DM1) is a common form of muscular dystrophy. Although several animal models have been established for DM1, myoblast cell models are still important because they offer an efficient cellular alternative for studying cellular and molecular events. Though C2C12 myoblast cells have been widely used to study myogenesis, resistance to gene transfection, or viral transduction, hinders research in C2C12 cells. Here, we describe an optimized protocol that includes daily maintenance, transfection and transduction procedures to introduce genes into C2C12 myoblasts and the induction of myocyte differentiation. Collectively, these procedures enable best transfection/transduction efficiencies, as well as consistent differentiation outcomes. The protocol described in establishing DM1 myoblast cell models would benefit the study of myotonic dystrophy, as well as other muscular diseases.
- Subjects :
- 0301 basic medicine
musculoskeletal diseases
Cellular differentiation
General Chemical Engineering
Biology
Transfection
Myotonic dystrophy
General Biochemistry, Genetics and Molecular Biology
Cell Line
Myoblasts
03 medical and health sciences
Transduction (genetics)
Mice
medicine
Myocyte
Animals
Myotonic Dystrophy
Muscular dystrophy
General Immunology and Microbiology
Myogenesis
General Neuroscience
Cell Differentiation
medicine.disease
musculoskeletal system
Molecular biology
Cell biology
030104 developmental biology
Medicine
C2C12
tissues
Subjects
Details
- ISSN :
- 1940087X
- Issue :
- 113
- Database :
- OpenAIRE
- Journal :
- Journal of visualized experiments : JoVE
- Accession number :
- edsair.doi.dedup.....9ac35c5aacd088c0c7ee0884ec197ded