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FTIR spectral signatures of mouse antral oocytes: Molecular markers of oocyte maturation and developmental competence
FTIR spectral signatures of mouse antral oocytes: Molecular markers of oocyte maturation and developmental competence
- Publication Year :
- 2011
- Publisher :
- Elsevier, 2011.
-
Abstract
- Mammalian antral oocytes with a Hoescht-positive DNA ring around the nucleolus (SN) are able to resume meiosis and to fully support the embryonic development, while oocytes with a non-surrounded nucleolus (NSN) cannot. Here, we applied FTIR microspectroscopy to characterize single SN and NSN mouse oocytes in order to try to elucidate some aspects of the mechanisms behind the different chromatin organization that impairs the full development of NSN oocyte-derived embryos. To this aim, oocytes were measured at three different stages of their maturation: just after isolation and classification as SN and NSN oocytes (time 0); after 10h of in vitro maturation, i.e. at the completion of the metaphase I (time 1); and after 20h of in vitro maturation, i.e. at the completion of the metaphase II (time 2). Significant spectral differences in the lipid (3050-2800cm-1) and protein (1700-1600cm-1) absorption regions were found between the two types of oocytes and among the different stages of maturation within the same oocyte type. Moreover, dramatic changes in nucleic acid content, concerning mainly the extent of transcription and polyadenylation, were detected in particular between 1000 and 800cm-1. The use of the multivariate principal component-linear discriminant analysis (PCA-LDA) enabled us to identify the maturation stage in which the separation between the two types of oocytes took place, finding as the most discriminating wavenumbers those associated to transcriptional activity and polyadenylation, in agreement with the visual analysis of the spectral data. © 2011 Elsevier B.V.
- Subjects :
- Time Factors
Polyadenylation
Nucleolus
Biology
Mice
Meiosis
Ovarian Follicle
Spectroscopy, Fourier Transform Infrared
medicine
Animals
developmental competence
FTIR microspectroscopy
Molecular Biology
Infrared spectroscopy
Genetics
Principal Component Analysis
SN oocytes
Embryogenesis
Discriminant Analysis
Proteins
Embryo
DNA
Cell Biology
Oocyte
Lipids
BIO/10 - BIOCHIMICA
In vitro maturation
Cell biology
Chromatin
NSN oocytes
medicine.anatomical_structure
oocyte maturation
FIS/01 - FISICA SPERIMENTALE
Multivariate Analysis
Oocytes
Female
Cell Nucleolus
BIO/05 - ZOOLOGIA
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....9af298494cf13a49bee04e3a90914879