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Polyubiquitylation of AMF requires cooperation between the gp78 and TRIM25 ubiquitin ligases
- Source :
- Oncotarget
- Publication Year :
- 2013
- Publisher :
- Impact Journals, LLC, 2013.
-
Abstract
- // Ying Wang 1,* , Seung-Wook Ha 1,* , Tianpeng Zhang 1,* , Dhong-Hyo Kho 1 , Avraham Raz 1 , and Youming Xie 1 1 Barbara Ann Karmanos Cancer Institute and Department of Oncology, Wayne State University School of Medicine, Detroit, MI, USA * These authors contributed equally to the work Correspondence: Avraham Raz, email: // Youming Xie, email: // Keywords : protein ubiquitylation, ubiquitin ligase, AMF, gp78, TRIM25 Received : October 8, 2013 Accepted : October 27, 2013 Published : October 28, 2013 Abstract gp78 is a ubiquitin ligase that plays a vital role in endoplasmic reticulum (ER)-associated degradation (ERAD). Here we report that autocrine motility factor (AMF), also known as phosphoglucose isomerase (PGI), is a novel substrate of gp78. We show that polyubiquitylation of AMF requires cooperative interaction between gp78 and the ubiquitin ligase TRIM25 (tripartite motif-containing protein 25). While TRIM25 mediates the initial round of ubiquitylation, gp78 catalyzes polyubiquitylation of AMF. The E4-like activity of gp78 was illustrated by an in vitro polyubiquitylation assay using Ub-DHFR as a model substrate. We further demonstrate that TRIM25 ubiquitylates gp78 and that overexpression of TRIM25 accelerates the degradation of gp78. Our data suggest that TRIM25 not only cooperates with gp78 in polyubiquitylation of AMF but also gauges the steady-state level of gp78. This study uncovers a previously unknown functional link between gp78 and TRIM25 and provides mechanistic insight into gp78-mediated protein ubiquitylation.
- Subjects :
- TRIM25
Immunoprecipitation
Ubiquitin-Protein Ligases
Immunoblotting
protein ubiquitylation
Endoplasmic-reticulum-associated protein degradation
ubiquitin ligase
Tripartite Motif Proteins
gp78
03 medical and health sciences
0302 clinical medicine
Ubiquitin
AMF
Humans
Transcription factor
030304 developmental biology
Genetics
0303 health sciences
biology
fungi
Glucose-6-Phosphate Isomerase
Ubiquitination
3. Good health
Ubiquitin ligase
Receptors, Autocrine Motility Factor
HEK293 Cells
Oncology
030220 oncology & carcinogenesis
biology.protein
Research Paper
Transcription Factors
Subjects
Details
- ISSN :
- 19492553
- Volume :
- 5
- Database :
- OpenAIRE
- Journal :
- Oncotarget
- Accession number :
- edsair.doi.dedup.....9b0ff105d06a4f31718a8637afea0b90