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Polydatin Restores Endothelium-Dependent Relaxation in Rat Aorta Rings Impaired by High Glucose: A Novel Insight into the PPARβ-NO Signaling Pathway
- Source :
- PLoS ONE, PLoS ONE, Vol 10, Iss 5, p e0126249 (2015)
- Publication Year :
- 2015
- Publisher :
- Public Library of Science (PLoS), 2015.
-
Abstract
- Polydatin, a natural component from Polygonum Cuspidatum, has important therapeutic effects on metabolic syndrome. A novel therapeutic strategy using polydatin to improve vascular function has recently been proposed to treat diabetes-related cardiovascular complications. However, the biological role and molecular basis of polydatin's action on vascular endothelial cells (VECs)-mediated vasodilatation under diabetes-related hyperglycemia condition remain elusive. The present study aimed to assess the contribution of polydatin in restoring endothelium-dependent relaxation and to determine the details of its underlying mechanism. By measuring endothelium-dependent relaxation, we found that acetylcholine-induced vasodilation was impaired by elevated glucose (55 mmol/L); however, polydatin (1, 3, 10 μmol/L) could restore the relaxation in a dose-dependent manner. Polydatin could also improve the histological damage to endothelial cells in the thoracic aorta. Polydatin's effects were mediated via promoting the expression of endothelial NO synthase (eNOS), enhancing eNOS activity and decreasing the inducible NOS (iNOS) level, finally resulting in a beneficial increase in NO release, which probably, at least in part, through activation of the PPARβ signaling pathway. The results provided a novel insight into polydatin action, via PPARβ-NO signaling pathways, in restoring endothelial function in high glucose conditions. The results also indicated the potential utility of polydatin to treat diabetes related cardiovascular diseases.
- Subjects :
- Male
medicine.medical_specialty
Nitric Oxide Synthase Type III
Endothelium
Vasodilator Agents
lcsh:Medicine
Nitric Oxide Synthase Type II
Aorta, Thoracic
Vasodilation
Pharmacology
Nitric Oxide
Nitric oxide
Rats, Sprague-Dawley
chemistry.chemical_compound
Enzyme activator
Glucosides
Enos
Fallopia japonica
medicine.artery
Internal medicine
Stilbenes
medicine
Animals
Thoracic aorta
lcsh:Science
PPAR-beta
Multidisciplinary
biology
business.industry
lcsh:R
Endothelial Cells
biology.organism_classification
Rats
Enzyme Activation
Glucose
medicine.anatomical_structure
Endocrinology
chemistry
Hyperglycemia
lcsh:Q
Female
Plant Preparations
Signal transduction
business
Signal Transduction
Research Article
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- PLOS ONE
- Accession number :
- edsair.doi.dedup.....9b2c5978f2f1cba53f50effd27134b30