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Shedding Light on Proton and Electron Dynamics in [FeFe] Hydrogenases
- Publication Year :
- 2020
-
Abstract
- [FeFe] hydrogenases are highly efficient catalysts for reversible dihydrogen evolution. H2 turnover involves different catalytic intermediates including a recently characterized hydride state of the active site (H-cluster). Applying cryogenic infrared and electron paramagnetic resonance spectroscopy to an [FeFe] model hydrogenase from Chlamydomonas reinhardtii (CrHydA1), we have discovered two new hydride intermediates and spectroscopic evidence for a bridging CO ligand in two reduced H-cluster states. Our study provides novel insights into these key intermediates, their relevance for the catalytic cycle of [FeFe] hydrogenase, and novel strategies for exploring these aspects in detail.
Details
- Language :
- English
- ISSN :
- 15205126
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....9b61c9ffc625aecc2c0da40988cd822e