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The presence of Ca(2+)-independent phospholipase A1 highly specific for phosphatidylinositol in bovine brain
- Source :
- Biochemical and biophysical research communications. 195(3)
- Publication Year :
- 1993
-
Abstract
- EDTA-insensitive phospholipase A activity hydrolyzing phosphatidylinositol was detected in a bovine brain soluble fraction. This phospholipase A was purified 25-fold by sequential chromatographies of DEAE-Toyopearl, Phenyl-Toyopearl, and Ultrahydrogel 1000. The partially purified EDTA-insensitive phospholipase A showed an apparent molecular mass of 230kDa on an Ultrahydrogel 1000 column in the presence of 0.05% Triton X-100 and a pH optimum at 7.0. The enzyme was highly specific for phosphatidylinositol, phosphatidylethanolamine and phosphatidylcholine were not hydrolyzed significantly. The enzyme activity was characterized as phospholipase A 1 , and Ca 2+ and Mg 2+ were not required for its activity. These results indicate the existence of Ca 2+ -independent, phosphatidylinositol-specific metabolism besides those catalyzed by Ca 2+ -dependent phospholipase A 2 and Ca 2+ -dependent, phosphatidylinositol-specific phospholipase C.
- Subjects :
- PLCD3
PLCB2
Biophysics
Magnesium Chloride
Biology
Phospholipase
Phosphatidylinositols
Biochemistry
Phospholipases A
Substrate Specificity
chemistry.chemical_compound
Calcium Chloride
Phospholipase A1
Phosphoinositide phospholipase C
Animals
Phosphatidylinositol
Molecular Biology
Edetic Acid
Phospholipase A
Phospholipase B
Hydrolysis
Brain
Cell Biology
Phospholipases A1
Phospholipases A2
chemistry
Calcium
Cattle
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 195
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Biochemical and biophysical research communications
- Accession number :
- edsair.doi.dedup.....9b631cab1dfbbc59e72bfcea6cb40393