Back to Search
Start Over
MicroRNA-based host response to toxicant exposure is influenced by the presence of gut microbial populations
- Source :
- Sci Total Environ
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- Segmented filamentous bacteria (SFB) and Bacteroides fragilis are known to interact with the host immune response through the aryl hydrocarbon receptor (Ahr). 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD), an environmental toxicant and a high-affinity Ahr ligand has the potential to modify the effect of SFB and B. fragilis. MicroRNAs (miRNA) with their role in regulating gene expression post-transcriptionally, may potentially be used to observe such interactions between SFB, B. fragilis, and TCDD. However, little is known regarding the impact of gut microbial members on miRNA expression or its modulation in the presence of an environmental toxicant. This information is important in understanding toxicant-mediated dysbiosis in gut microbiome and the resulting human health impacts. In this study, C57BL/6 germ-free (GF) mice were colonized with SFB and B. fragilis and administered 30 μg/kg TCDD every 4 d for 28 d and miRNA were measured. Compared to GF mice, colonization with SFB resulted in an increase in up- and down-regulated Ileal miRNAs. TCDD treatment of this group decreased the number of upregulated miRNA and increased the number of down-regulated miRNAs. Association with SFB and B. fragilis together had a similar but less pronounced effect in response to TCDD treatment. TCDD treatment of GF mice had no miRNA expression response. Immune and inflammatory responses and T-cell differentiation were the key functions impacted by these miRNAs. Overall, these results reveal that the host response to toxicants may also depend on the presence of specific gut microbial populations.
- Subjects :
- Polychlorinated Dibenzodioxins
Environmental Engineering
Segmented filamentous bacteria
Article
Microbiology
Mice
chemistry.chemical_compound
Immune system
Downregulation and upregulation
Gene expression
medicine
Animals
Environmental Chemistry
Waste Management and Disposal
biology
Immunity
medicine.disease
Aryl hydrocarbon receptor
biology.organism_classification
Pollution
Gastrointestinal Microbiome
Mice, Inbred C57BL
MicroRNAs
Receptors, Aryl Hydrocarbon
chemistry
biology.protein
Bacteroides fragilis
Dysbiosis
Toxicant
Subjects
Details
- ISSN :
- 00489697
- Volume :
- 797
- Database :
- OpenAIRE
- Journal :
- Science of The Total Environment
- Accession number :
- edsair.doi.dedup.....9b7e1a6d26f30701f12a8c5ebb19fba4