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Leptocarpin Suppresses Proliferation, Migration, and Invasion of Human Osteosarcoma by Targeting Type-1 Insulin-Like Growth Factor Receptor (IGF-1R)
- Source :
- Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
- Publication Year :
- 2017
- Publisher :
- International Scientific Literature, Inc., 2017.
-
Abstract
- BACKGROUND Leptocarpin (LTC) has drawn much attention for suppressing tumor growth or reducing inflammation. However, the effect of LTC on osteosarcoma has rarely been reported. Our object was to determine whether LTC suppresses MG63 cell proliferation, migration, and invasion, and whether type-1 insulin-like growth factor receptor (IGF-1R) is one of the targets in LTC suppressing osteosarcoma. MATERIAL AND METHODS Cytotoxicity of LTC was performed by use of a cell-counting kit-8 (CCK-8). RNA interference (RNAi) or pEABE-bleo IGF-1R plasmid were used for silencing or overexpressing IGF-1R, Western blot (WB) analysis was used for IGF-1R expression, CCK-8 for proliferation, and transwell assay for migration and invasion. RESULTS LTC (23.533 μM) treatment for 48 h was taken as the 50% inhibiting concentration (IC50), which significantly (P
- Subjects :
- 0301 basic medicine
Small interfering RNA
Down-Regulation
Apoptosis
Bone Neoplasms
Insulin-Like Growth Factor Receptor
Receptor, IGF Type 1
03 medical and health sciences
0302 clinical medicine
Growth factor receptor
Lab/In Vitro Research
Cell Movement
Cell Line, Tumor
Gene silencing
Humans
RNA, Small Interfering
Cell Proliferation
Osteosarcoma
Cell growth
Chemistry
Cell Migration Inhibition
Receptors, Somatomedin
General Medicine
Transfection
MicroRNAs
030104 developmental biology
Cell culture
030220 oncology & carcinogenesis
Cancer research
RNA Interference
Sesquiterpenes
Signal Transduction
Subjects
Details
- Language :
- English
- ISSN :
- 16433750 and 12341010
- Volume :
- 23
- Database :
- OpenAIRE
- Journal :
- Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
- Accession number :
- edsair.doi.dedup.....9b9607ed89b6e0725a92f63aa30c4fd4