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Direct comparison of vasectomized males and genetically sterile Gapdhs knockout males for the induction of pseudopregnancy in mice
- Source :
- Laboratory Animals. 52:365-372
- Publication Year :
- 2017
- Publisher :
- SAGE Publications, 2017.
-
Abstract
- The laboratory mouse is the most used animal model in biomedical research. Several artificial reproductive techniques, such as revitalization of cryopreserved strains, rederivation after hygienic contaminations and the production of transgenic mouse models, require the transfer of preimplantation embryos to surrogate mothers. Pseudopregnancy is essential in recipient females and is induced by mating with sterile males. Commonly, surgically vasectomized males are used for this purpose. As an alternative, genetically modified mouse strains have been identified, in which homozygous infertile males are sexually active. Here, we investigated the suitability of genetically infertile Gapdhstm1Dao males under routine laboratory conditions with respect to plug rates, pregnancy rates and frequency of born offspring after embryo transfer. Our results showed no significant differences for these aspects between Gapdhstm1Dao and vasectomized CD2F1 males. In addition, we evaluated the efforts to obtain a defined number of sterile males either by breeding of sterile mutants or surgical vasectomy, and addressed the impact of both options on animal welfare. In conclusion, infertile males of the Gapdhstm1Dao line are a reliable alternative to vasectomized males for the induction of pseudopregnancy, and can contribute to the refinement of the procedure by avoiding surgical interventions.
- Subjects :
- Male
0301 basic medicine
Genetically modified mouse
Pregnancy Rate
Offspring
Physiology
Biology
Cryopreservation
Mice
03 medical and health sciences
Pregnancy
Vasectomy
medicine
Animals
Pseudopregnancy
Mating
Infertility, Male
Mice, Knockout
Mice, Inbred BALB C
General Veterinary
Laboratory mouse
Embryo Transfer
medicine.disease
Embryo transfer
030104 developmental biology
Mice, Inbred DBA
Models, Animal
Female
Animal Science and Zoology
Subjects
Details
- ISSN :
- 17581117 and 00236772
- Volume :
- 52
- Database :
- OpenAIRE
- Journal :
- Laboratory Animals
- Accession number :
- edsair.doi.dedup.....9bb9928c2daecca886c393b87b23331f