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HPLC-MS/MS method to measure trabectedin in tumors: Preliminary PK study in a mesothelioma xenograft model
- Publication Year :
- 2015
- Publisher :
- Future Science Ltd, 2015.
-
Abstract
- Background: Trabectedin is an anticancer agent registered for the second-line treatment of soft tissue sarcoma and ovarian cancer. No preclinical data are available on its tumor distribution, so a method for quantification in neoplastic tissues is required. Methods/results: We validated an LC–MS/MS assay determining the recovery, sensitivity, linearity, precision and accuracy in mouse tumor and liver samples. The limit of quantification was 0.10 ng/ml with a curve range of 0.10–3.00 ng/ml (accuracy 96.1–102.1%). Inter-day precision and accuracy of QCs were 6.0–8.2 and 97.0–102.6% respectively. The method was applied in mesothelioma xenografts treated with therapeutic doses. Conclusion: The method was validated for measuring trabectedin in tissues. In a mesothelioma xenograft model, trabectedin distributed preferentially in tumor compared with liver.
- Subjects :
- Mesothelioma
Spectrometry, Mass, Electrospray Ionization
Clinical Biochemistry
Analytical chemistry
Mice, Nude
Dioxoles
Analytical Chemistry
Mice
Tandem Mass Spectrometry
Tetrahydroisoquinolines
medicine
Distribution (pharmacology)
Animals
Humans
Mouse tumor
General Pharmacology, Toxicology and Pharmaceutics
Antineoplastic Agents, Alkylating
Animals, Antineoplastic Agents, Alkylating, Chromatography, High Pressure Liquid, Dioxoles, Female, Humans, Mesothelioma, Mice, Nude, Spectrometry, Mass, Electrospray Ionization, Tandem Mass Spectrometry, Tetrahydroisoquinolines, Trabectedin, Xenograft Model Antitumor Assays
Trabectedin
Chromatography, High Pressure Liquid
Detection limit
Chemistry
Soft tissue sarcoma
General Medicine
medicine.disease
Xenograft Model Antitumor Assays
Medical Laboratory Technology
Hplc ms ms
Cancer research
Female
Ovarian cancer
medicine.drug
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....9bea80d53cbfc5b35bb0851546ac3cfb