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Degenerate oligonucleotide primers for enzymatic amplification of recA sequences from gram-positive bacteria and mycoplasmas
- Publication Year :
- 1992
-
Abstract
- RecA protein in gram-negative bacteria, especially in Escherichia coli, has been extensively studied, but little is known about this key enzyme in other procaryotes. Described here are degenerate oligonucleotide primers that have been used to amplify by the polymerase chain reaction (PCR) recA sequences from several gram-positive bacteria and mycoplasmas. The DNA sequences of recA PCR products from Streptococcus pyogenes, Streptococcus mutans, Enterococcus faecalis, and Mycoplasma pulmonis were determined and compared. These data indicate that the M. pulmonis recA gene has diverged significantly from recA genes of other eubacteria. It should be possible to use cloned recA PCR products to construct recA mutants, thereby providing the means of elucidating homologous genetic recombination and DNA repair activities in these organisms.
- Subjects :
- DNA, Bacterial
DNA repair
Molecular Sequence Data
Biology
medicine.disease_cause
Microbiology
Genetic recombination
Polymerase Chain Reaction
law.invention
chemistry.chemical_compound
Mycoplasma
Species Specificity
law
medicine
Amino Acid Sequence
Molecular Biology
Escherichia coli
Polymerase chain reaction
Bacteria
Base Sequence
Oligonucleotide
biochemical phenomena, metabolism, and nutrition
Molecular biology
Rec A Recombinases
chemistry
Oligodeoxyribonucleotides
Genes, Bacterial
Mycoplasma pulmonis
bacteria
Primer (molecular biology)
Sequence Alignment
DNA
Research Article
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....9bf47a0f138e983a48638af80559e639